Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, 3584 CL, Utrecht, The Netherland.
Vet Rec. 2011 Jun 4;168(22):587. doi: 10.1136/vr.d1091. Epub 2011 May 24.
Settled dust samples were collected on a commercial dairy farm in the Netherlands with a high prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) (barn A) and on a Dutch experimental cattle farm (barn B) stocked with cattle confirmed to be MAP shedders. Barns were sampled while animals were present, after both barns were destocked and cleaned by cold high-pressure cleaning, and after being kept empty for two weeks (barn A) or after additional disinfection (barn B). MAP DNA was detected by IS900 real-time PCR and viable MAP were detected by liquid culture. MAP DNA was detected in 78 per cent of samples from barn A and 86 per cent of samples from barn B collected while animals were still present. Viable MAP was detected in six of nine samples from barn A and in three of seven samples from barn B. After cold high-pressure cleaning, viable MAP could be detected in only two samples from each barn. After leaving barn A empty for two weeks, and following additional disinfection of barn B, no viable MAP could be detected in any settled dust sample.
在荷兰一个奶牛场（畜棚 A）采集了定居尘埃样本，该奶牛场的分枝杆菌副结核亚种（MAP）流行率很高，还在一个荷兰实验性奶牛场（畜棚 B）采集了样本，该奶牛场的牛被确认为 MAP 排出者。在动物在场的情况下、畜棚清空和清洁（畜棚 A）或经过额外消毒（畜棚 B）后进行采样。通过 IS900 实时 PCR 检测 MAP DNA，通过液体培养检测活的 MAP。在畜棚 A 仍有动物时采集的 78%的样本和畜棚 B 采集的 86%的样本中检测到 MAP DNA。在畜棚 A 的 9 个样本中的 6 个和畜棚 B 的 7 个样本中的 3 个样本中检测到活的 MAP。经过冷高压清洗后，每个畜棚只能从两个样本中检测到活的 MAP。畜棚 A 闲置两周后，对畜棚 B 进行了额外消毒，在任何定居尘埃样本中均未检测到活的 MAP。
