Department of Geriatrics, The Affiliated Zhongshan Hospital of Fudan University, Shanghai, 200032, China.
Target Oncol. 2011 Sep;6(3):147-54. doi: 10.1007/s11523-011-0182-9. Epub 2011 May 25.
The aim of the study was to observe the variation of CD4(+)CD25(+) regulatory T cells in periphery blood and tumor microenvironment of non-small cell lung cancer (NSCLC) patients and the effects of CpG ODN. The proportion of CD4(+)CD25(+) regulatory T cells, Foxp3 gene expression, levels of tumor growth factor-β (TGF-β) and immunoreactive fibronectin-γ (IFN-γ) in the periphery blood of 30 NSCLC patients and 30 healthy volunteers were compared. These indicators were compared before and after CpG ODN treatment. Foxp3 gene expression in the tumor microenvironment of NSCLC patients was also observed. The results showed CD4(+)CD25(+) regulatory T cell proportion, Foxp3 expression and TGF-β levels in the periphery blood of NSCLC patients were higher than those of healthy volunteers (p < 0.05), and these indicators of patients were significantly decreased after CpG ODN 2006 treatment (p < 0.05). Foxp3 expression in the metastatic lymph nodes was higher than that in the non-metastatic ones of NSCLC patients (p = 0.000). In conclusion, a rise in the proportion of CD4(+)CD25(+)Foxp3(+) regulatory T cells was demonstrated in the periphery blood and tumor microenvironments of NSCLC patients. CpG ODN 2006 downregulated the CD4(+)CD25(+)Foxp3(+) regulatory T cells proportion and TGF-β levels in the periphery blood of these patients.
本研究旨在观察非小细胞肺癌（NSCLC）患者外周血和肿瘤微环境中 CD4+CD25+调节性 T 细胞的变化及 CpG ODN 的作用。比较 30 例 NSCLC 患者和 30 例健康志愿者外周血中 CD4+CD25+调节性 T 细胞的比例、Foxp3 基因表达、肿瘤生长因子-β（TGF-β）和免疫反应性纤维连接蛋白-γ（IFN-γ）水平，观察 CpG ODN 治疗前后这些指标的变化，并观察 NSCLC 患者肿瘤微环境中 Foxp3 基因的表达。结果显示，NSCLC 患者外周血中 CD4+CD25+调节性 T 细胞比例、Foxp3 表达和 TGF-β水平均高于健康志愿者（p<0.05），经 CpG ODN 2006 治疗后，这些指标均显著降低（p<0.05）。Foxp3 表达在 NSCLC 患者转移淋巴结中高于非转移淋巴结（p=0.000）。结论：NSCLC 患者外周血和肿瘤微环境中 CD4+CD25+Foxp3+调节性 T 细胞比例升高，CpG ODN 2006 可下调此类患者外周血中 CD4+CD25+Foxp3+调节性 T 细胞的比例和 TGF-β水平。
