肿瘤坏死因子-α对维甲酸耐药细胞系U937/GTB的细胞毒性作用及环磷酸腺苷的异常调节
Cytotoxic effect of TNF-alpha and abnormal regulation of cAMP in the retinoid resistant cell line U937/GTB.
作者信息
Botilsrud M, Kvam L, Blomhoff H K, Norum K R, Blomhoff R
机构信息
Institute for Nutrition Research, School of Medicine, University of Oslo, Norway.
出版信息
Anticancer Res. 1990 Mar-Apr;10(2A):433-6.
We have studied the effect of retinoids, forskolin and TNF-alpha on two sublines of U937 cells, U937/clone 4 and U937/GTB. Retinoids induced differentiation of U937/clone 4, while the U937/GTB cells were resistant to induction of differentiation by retinoids. Retinoids effectively reduced the proliferation of both U937/clone 4 and U937/GTB, and decreased the steady state levels of c-myc mRNA in both sublines. Furthermore, the retinoid resistant U937/GTB cells were hypersensitive to the cytotoxic effect of TNF-alpha. Forskolin increased the cAMP level in the U937/clone 4 cells 14 times above basal level, but did not change the cAMP concentration in the U937/GTB cells.
我们研究了类视黄醇、福斯高林和肿瘤坏死因子-α（TNF-α）对U937细胞的两个亚系U937/克隆4和U937/GTB的影响。类视黄醇诱导U937/克隆4分化，而U937/GTB细胞对类视黄醇诱导的分化具有抗性。类视黄醇有效降低了U937/克隆4和U937/GTB的增殖，并降低了两个亚系中c-myc mRNA的稳态水平。此外，对类视黄醇耐药的U937/GTB细胞对TNF-α的细胞毒性作用高度敏感。福斯高林使U937/克隆4细胞中的环磷酸腺苷（cAMP）水平比基础水平升高了14倍，但未改变U937/GTB细胞中的cAMP浓度。
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