The Key Sericultural Laboratory of Agricultural Ministry, Southwest University, Chongqing, PR China.
DNA Cell Biol. 2011 Oct;30(10):763-70. doi: 10.1089/dna.2011.1224. Epub 2011 May 25.
The flap endonuclease-1 (FEN-1) gene is involved in DNA replication and repair, and it maintains genomic stability as well as the accuracy of DNA replication under normal growth conditions. However, FEN-1 also plays an important role in apoptosis and cancer development. We cloned the BmFEN-1 gene from Bombyx mori, which was 1343 bp in length and possessed an 1143 bp ORF (123-1266). It consists of seven introns and eight exons that encode a protein with 380 amino acids that has the typical XPG domain. The N-terminal motif is located at amino acids 95-105, and the proliferating cell nuclear antigen interaction motif is located at amino acids 337-344. RNA interference-mediated reduction of BmFEN-1 expression induced cell cycle arrest in S phase in BmE-SWU1 cells. These results suggest that BmFEN-1 can inhibit apoptosis and promote cell proliferation.
Flap endonuclease-1 (FEN-1) 基因参与 DNA 复制和修复，在正常生长条件下维持基因组稳定性和 DNA 复制的准确性。然而，FEN-1 在细胞凋亡和癌症发展中也起着重要作用。我们从家蚕中克隆了 BmFEN-1 基因，该基因长 1343bp，具有 1143bp 的 ORF（123-1266）。它由七个内含子和八个外显子组成，编码一个具有 380 个氨基酸的蛋白质，具有典型的 XPG 结构域。N 端基序位于氨基酸 95-105，增殖细胞核抗原相互作用基序位于氨基酸 337-344。BmFEN-1 的 RNA 干扰介导的表达降低导致 BmE-SWU1 细胞的 S 期细胞周期停滞。这些结果表明，BmFEN-1 可以抑制细胞凋亡并促进细胞增殖。
