RUNX3 在卵巢癌中作为癌基因发挥作用。
RUNX3 functions as an oncogene in ovarian cancer.
机构信息
Cancer Science Institute of Singapore, National University of Singapore, Singapore.
出版信息
Gynecol Oncol. 2011 Aug;122(2):410-7. doi: 10.1016/j.ygyno.2011.04.044. Epub 2011 May 25.
OBJECTIVE
The Runt domain transcription factor, RUNX3, has been shown to be a tumor suppressor in a variety of cancers including gastric, colon and breast cancer. Interestingly, an oncogenic role for RUNX3 has also been suggested in basal cell carcinoma and head and neck cancer. Here, we explore the role of RUNX3 in ovarian cancer.
METHODS
Expression of RUNX3 mRNA and protein was evaluated in human ovarian cancer cell lines. In addition, subcellular localization of RUNX3 was also examined in cell lines and ovarian cancer tissues. Effect of exogenous RUNX3 expression and knockdown on cell proliferation was investigated by proliferation assays and a soft agar assay.
RESULTS
Expression of RUNX3 was detected in the nucleus of ovarian cancer cell lines and ovarian cancer tissues and was found to play a growth stimulatory role. RUNX3 knockdown resulted in a decrease in cell proliferation in liquid media as well as in soft agar. Despite the fact that exogenous expression of RUNX3 strongly inhibits cell growth in many cell types, RUNX3 promoted cell growth in ovarian cancer cell lines not expressing RUNX3.
CONCLUSION
RUNX3 is frequently expressed in the nuclei of ovarian cancer cell lines and plays an oncogenic role in ovarian cancer.
目的
runt 结构域转录因子 runt3 已被证实是多种癌症（包括胃癌、结肠癌和乳腺癌）的肿瘤抑制因子。有趣的是，runt3 在基底细胞癌和头颈部癌症中也具有致癌作用。在这里，我们探讨了 runt3 在卵巢癌中的作用。
方法
在人卵巢癌细胞系中评估 runt3 mRNA 和蛋白的表达。此外，还在细胞系和卵巢癌组织中检查了 runt3 的亚细胞定位。通过增殖试验和软琼脂试验研究外源性 runt3 表达和敲低对细胞增殖的影响。
结果
在卵巢癌细胞系和卵巢癌组织的细胞核中检测到 runt3 的表达，并且发现其发挥生长刺激作用。runx3 敲低导致液体培养基和软琼脂中的细胞增殖减少。尽管外源性表达 runt3 强烈抑制许多细胞类型的细胞生长，但 runt3 促进不表达 runt3 的卵巢癌细胞系的细胞生长。
结论
runt3 常在卵巢癌细胞系的细胞核中表达，并在卵巢癌中发挥致癌作用。
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