Szmajda-Krygier Dagmara, Krygier Adrian, Jamroziak Krzysztof, Korycka-Wołowiec Anna, Żebrowska-Nawrocka Marta, Balcerczak Ewa
Laboratory of Molecular Diagnostics and Pharmacogenomics, Department of Pharmaceutical Biochemistry and Molecular Diagnostics, Medical University of Lodz, Muszynskiego 1 Street, 90-151 Lodz, Poland.
Department of Hematology, Institute of Hematology and Transfusion Medicine, Chocimska 5 Street, 00-791 Warsaw, Poland.
Curr Issues Mol Biol. 2022 Aug 1;44(8):3455-3464. doi: 10.3390/cimb44080238.
The genetic factors of adult acute lymphoblastic leukemia (ALL) development are only partially understood. The Runt-Related Transcription Factor () gene family play a crucial role in hematological malignancies, serving both a tumor suppressor and promoter function. The aim of this study was the assessment of relative and genes expression level among adult ALL cases and a geographically and ethnically matched control group. The relative and genes expression level was assessed by qPCR. The investigated group comprised 60 adult patients newly diagnosed with ALL. The obtained results were compared with a group of 40 healthy individuals, as well as clinical and hematological parameters of patients, and submitted for statistical analysis. ALL patients tend to have significantly higher gene expression level compared with controls. This observation is also true for risk group stratification where high-risk (HR) patients presented higher levels of . A higher transcript level correlates with greater leukocytosis while expression is reduced in Philadelphia chromosome bearers. The conducted study sustains the hypothesis that both a reduction and increase in the transcript level of family genes may be involved in leukemia pathogenesis, although their interaction is complex. In this context, overexpression of the gene in adult ALL cases in particular seems interesting. Obtained results should be interpreted with caution. Further analysis in this research field is needed.
成人急性淋巴细胞白血病（ALL）发生发展的遗传因素目前仅得到部分了解。 runt相关转录因子（）基因家族在血液系统恶性肿瘤中起着关键作用，兼具肿瘤抑制和促进功能。本研究旨在评估成人ALL病例与地理和种族匹配的对照组中相关基因和基因的表达水平。通过qPCR评估相关基因和基因的表达水平。研究组包括60例新诊断为ALL的成年患者。将所得结果与40名健康个体组成的组以及患者的临床和血液学参数进行比较，并进行统计分析。与对照组相比，ALL患者的基因表达水平往往显著更高。对于风险组分层也是如此，高危（HR）患者的水平更高。较高的转录本水平与更高的白细胞增多相关，而在费城染色体携带者中表达降低。本研究支持这样的假设，即基因家族基因转录水平的降低和升高都可能参与白血病的发病机制，尽管它们的相互作用很复杂。在这种情况下，特别是成人ALL病例中基因的过表达似乎很有趣。所得结果应谨慎解释。该研究领域需要进一步分析。
