MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China.
Biosens Bioelectron. 2011 Jul 15;26(11):4436-41. doi: 10.1016/j.bios.2011.04.059. Epub 2011 May 6.
A novel nitrite biosensor was developed through a sensing platform consisted of single-layer graphene nanoplatelet (SLGnP)-protein composite film. SLGnP with the virtues of excellent biocompatibility, conductivity and high sensitivity to the local perturbations can provide a biocompatible microenvironment for protein immobilization and a suitable electron transfer distance between electroactive centers of heme protein and electrode surface. A pair of well-defined and quasi-reversible cyclic voltammetric peaks that reflected the direct electrochemistry for ferric/ferrous couple of myoglobin (Mb) was achieved at the composite film modified electrode. Field emission scanning electron microscopy (FESEM) and ultraviolet visible spectra (UV-vis) were utilized to characterize the composite film. The results demonstrated that the morphology of the composite film was unique and the protein in the composite film retained its secondary structure similar to the native state. The composite film also displayed excellent electrocatalytic ability for the reduction of nitric oxide, which was applied to determine nitrite indirectly. It exhibited good electrochemical response to nitrite with a linear range from 0.05 to 2.5 mM and a detection limit of 0.01 mM.
一种新型的亚硝酸盐生物传感器是通过一个由单层石墨烯纳米片（SLGnP）-蛋白质复合膜组成的传感平台开发的。SLGnP 具有优异的生物相容性、导电性和对局部扰动的高灵敏度，可以为蛋白质固定化提供一个生物相容的微环境，并为血红素蛋白的电化学活性中心与电极表面之间提供合适的电子转移距离。在复合膜修饰电极上实现了一对定义良好且近乎可逆的循环伏安峰，反映了肌红蛋白（Mb）的铁/亚铁对的直接电化学。场发射扫描电子显微镜（FESEM）和紫外可见光谱（UV-vis）用于表征复合膜。结果表明，复合膜的形态独特，并且复合膜中的蛋白质保留了与其天然状态相似的二级结构。复合膜对一氧化氮的还原也表现出优异的电催化能力，可用于间接测定亚硝酸盐。它对亚硝酸盐表现出良好的电化学响应，线性范围为 0.05 至 2.5 mM，检测限为 0.01 mM。
