Department of Chemistry, Research Institute for Basic Sciences, and Research Center for New Nano Bio Fusion Technology, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
Bioorg Med Chem Lett. 2011 Jul 1;21(13):4020-2. doi: 10.1016/j.bmcl.2011.04.139. Epub 2011 May 6.
A UO(2)(2+)-specific DNA aptamer was attached to aminopolystyrene (aminoPS) using sulfo-SMCC as a crosslinking agent in view of high affinity of DNA for uranyl ion. Capacity of the aptamer-conjugated aminoPS resins for uranyl uptake was measured, revealing that about 0.63 μg of uranium can be complexed to 1g of the resins, which clearly demonstrates that most of DNA aptamers introduced to the resins can strongly bind to uranyl ion. In the presence of 21 mM bicarbonate ion at pH 8.01, apparent dissociation constant (K(d)(app)) of about 84.6 pM and log formation constant (K(f)) of about 22.9 were obtained. Results of the present study strongly suggest that modification of the aptamer-containing resins can improve uranyl-binding ability, probably leading to economical recovery of uranium from seawater.
一种 UO(2)(2+)-特异性 DNA 适体通过磺基-SMCC 连接到氨基聚苯乙烯（aminoPS）上，因为 DNA 对铀酰离子具有高亲和力。测量了适体偶联的氨基 PS 树脂对铀酰的摄取能力，表明约 0.63 μg 的铀可以与 1g 的树脂络合，这清楚地表明引入树脂的大多数 DNA 适体可以与铀酰离子强烈结合。在 pH 值为 8.01 时存在 21mM 碳酸氢根离子的情况下，得到了约 84.6 pM 的表观离解常数（K(d)(app)）和约 22.9 的对数形成常数（K(f)）。本研究结果强烈表明，对含适体的树脂进行修饰可以提高铀酰结合能力，可能会导致从海水中经济地回收铀。
