Wu Jingjing, Zhu Yingyue, Xue Feng, Mei Zhanlong, Yao Li, Wang Xin, Zheng Lei, Liu Jian, Liu Guodong, Peng Chifang, Chen Wei
School of Biotechnology and Food Engineering, Anhui Provincial Key Lab of Functional Materials & Devices, Hefei University of Technology, Hefei 230009, People's Republic of China.
School of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu 215500, People's Republic of China.
Mikrochim Acta. 2014 Apr;181(5-6):479-491. doi: 10.1007/s00604-013-1156-7.
The method referred to as "systemic evolution of ligands by exponential enrichment" (SELEX) was introduced in 1990 and ever since has become an important tool for the identification and screening of aptamers. Such nucleic acids can recognize and bind to their corresponding targets (analytes) with high selectivity and affinity, and aptamers therefore have become attractive alternatives to traditional antibodies not the least because they are much more stable. Meanwhile, they have found numerous applications in different fields including food quality and safety monitoring. This review first gives an introduction into the selection process and to the evolution of SELEX, then covers applications of aptamers in the surveillance of food safety (with subsections on absorptiometric, electrochemical, fluorescent and other methods), and then gives conclusions and perspectives. The SELEX method excels by its features of in vitro, high throughput and ease of operation. This review contains 86 references.
1990年引入了一种称为“指数富集的配体系统进化”（SELEX）的方法，自那时起它已成为鉴定和筛选适体的重要工具。这类核酸能够以高选择性和亲和力识别并结合其相应的靶标（分析物），因此适体已成为传统抗体颇具吸引力的替代品，这尤其是因为它们更加稳定。同时，它们在包括食品质量与安全监测在内的不同领域已有众多应用。本综述首先介绍SELEX的筛选过程及其发展，接着涵盖适体在食品安全监测中的应用（包括吸光法、电化学法、荧光法及其他方法等小节），然后给出结论与展望。SELEX方法以其体外、高通量及操作简便的特点而见长。本综述包含86篇参考文献。
