Institute of Agricultural Biotechnology, 42 Timiryazevskaya ul., 127550 Moscow, Russia.
Am J Bot. 2011 Mar;98(3):e54-7. doi: 10.3732/ajb.1000422. Epub 2011 Mar 8.
Traditional taxonomy and nomenclature of Brassiceae (Brassicaceae) species do not reflect their phylogeny. Revision of the species and generic limits supported by extensive molecular data seems crucial.
Genome-specific polymorphisms extracted from non-coding and coding sequences were used to develop 14 sequence characterized amplified region (SCAR) markers specific for the Brassica B genome. These SCARs were verified against 77 accessions of six U-triangle Brassica species and used to screen 23 accessions of seven wild Brassiceae species to test for their cross-species amplification. SCARs were found in all B-genome Brassica species and also in Sinapis arvensis.
SCAR markers can be employed for discerning B-genome chromosomes in Brassica species and S. arvensis to reliably identify B-genome species and their natural hybrids. The combined molecular evidence supports the suggestion to revise the generic limits of Brassica and Sinapis.
传统的芸薹属（十字花科）物种分类和命名法不能反映其系统发育。修订由广泛的分子数据支持的物种和属的界限似乎至关重要。
从非编码和编码序列中提取的基因组特异性多态性用于开发 14 个序列特征扩增区域（SCAR）标记，这些标记特异性针对芸薹属 B 基因组。这些 SCAR 标记在六个 U 形三角芸薹属物种的 77 个材料上进行了验证，并用于筛选七个野生芸薹属物种的 23 个材料，以测试它们在种间的扩增情况。SCAR 标记在所有 B 基因组芸薹属物种以及白芥中都有发现。
SCAR 标记可用于区分芸薹属物种和白芥中的 B 基因组染色体，以可靠地鉴定 B 基因组物种及其天然杂种。综合分子证据支持修订芸薹属和白芥属的属界限的建议。
