Sairkar Pramod Kumar, Sharma Anjana, Shukla N P
Centre of Excellence in Biotechnology, M. P. Council of Science & Technology, Bhopal, Madhya Pradesh, India.
Bacteriology Laboratory, Department of Post Graduate Studies & Research in Biological Science, Rani Durgawati University, Jabalpur, Madhya Pradesh, India.
Mol Biol Int. 2016;2016:1482796. doi: 10.1155/2016/1482796. Epub 2016 Mar 16.
Commercially important Commiphora species are drought-tolerant plants and they are leafless for most of the year. Therefore, it is necessary to develop some molecular marker for the identification. Intended for that, in the present study, species-specific, sequence-characterized amplified regions (SCAR) markers were developed for proficient and precise identification of closely related species Commiphora wightii and C. myrrha, which may ensure the quality, safety, and efficacy of medicines made from these plants through adulterous mixing of these plants. Two species-specific RAPD amplicons were selected, gel-purified, cloned, and sequenced after screening of 20 RAPD primers. The sequence of 979 and 590 nucleotides (Genebank accession numbers K90051 and K90052) was used for development of 4 SCAR markers, namely, Sc1P, Sc1Pm, Sc2P, and Sc2Pm. Out of them, the Sc1Pm was specific for C. wightii, while Sc2P discriminated both the Commiphora species. These markers are first reported and will be useful for rapid identification of closely related Commiphora wightii and C. myrrha species.
具有商业重要性的没药属植物是耐旱植物，一年中大部分时间都没有叶子。因此，有必要开发一些分子标记用于鉴定。为此，在本研究中，开发了物种特异性的序列特征扩增区域（SCAR）标记，用于准确和精确地鉴定近缘物种没药树和没药，这可以确保由这些植物制成的药物在通过这些植物的掺假混合时的质量、安全性和有效性。在筛选了20个随机扩增多态性DNA（RAPD）引物后，选择了两个物种特异性的RAPD扩增子，进行凝胶纯化、克隆和测序。979和590个核苷酸的序列（基因库登录号K90051和K90052）用于开发4个SCAR标记，即Sc1P、Sc1Pm、Sc2P和Sc2Pm。其中，Sc1Pm对没药树具有特异性，而Sc2P可以区分这两种没药属植物。这些标记是首次报道，将有助于快速鉴定近缘的没药树和没药物种。
