The Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.
Am J Bot. 2011 Apr;98(4):e76-7. doi: 10.3732/ajb.1000446. Epub 2011 Mar 30.
Microsatellite primers were developed for the important and popular ornamental spring orchid, Cymbidium goeringii, to investigate its genetic diversity and population genetic structure.
Using the Fast Isolation by AFLP of Sequences Containing (FIASCO) repeats protocol, 15 primer sets were identified in two wild populations. The number of alleles per locus ranged from 2 to 5, with a mean of 3.3. The observed and expected heterozygosities varied from 0.167 to 0.917 and from 0.159 to 0.822, respectively. All these primers successfully amplified in the congener C. tortisepalum, and 12 primers were found useful in C. faberi and C. sinense.
These markers will facilitate further studies on the population genetics of Cymbidium goeringii and other congeneric species.
为了研究重要且受欢迎的观赏春兰（Cymbidium goeringii）的遗传多样性和种群遗传结构，我们开发了微卫星引物。
利用序列包含（FIASCO）重复的快速 AFLP 分离（Fast Isolation by AFLP of Sequences Containing，FIASCO）协议，在两个野生种群中鉴定出 15 对引物。每个位点的等位基因数范围为 2 到 5，平均值为 3.3。观察到的和预期的杂合度分别在 0.167 到 0.917 和 0.159 到 0.822 之间变化。所有这些引物都能成功地在近缘种 C. tortisepalum 中扩增，并且有 12 个引物在 C. faberi 和 C. sinense 中也很有用。
这些标记将有助于进一步研究春兰及其它近缘种的种群遗传学。
