从春兰（Cymbidium goeringii）（兰科）中分离和鉴定 15 个微卫星标记。

Isolation and characterization of 15 microsatellite markers from the spring orchid (Cymbidium goeringii) (Orchidaceae).

机构信息

The Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.

出版信息

Am J Bot. 2011 Apr;98(4):e76-7. doi: 10.3732/ajb.1000446. Epub 2011 Mar 30.

DOI:10.3732/ajb.1000446

PMID:21613151

Abstract

PREMISE OF THE STUDY

Microsatellite primers were developed for the important and popular ornamental spring orchid, Cymbidium goeringii, to investigate its genetic diversity and population genetic structure.

METHODS AND RESULTS

Using the Fast Isolation by AFLP of Sequences Containing (FIASCO) repeats protocol, 15 primer sets were identified in two wild populations. The number of alleles per locus ranged from 2 to 5, with a mean of 3.3. The observed and expected heterozygosities varied from 0.167 to 0.917 and from 0.159 to 0.822, respectively. All these primers successfully amplified in the congener C. tortisepalum, and 12 primers were found useful in C. faberi and C. sinense.

CONCLUSIONS

These markers will facilitate further studies on the population genetics of Cymbidium goeringii and other congeneric species.

摘要

研究前提

为了研究重要且受欢迎的观赏春兰（Cymbidium goeringii）的遗传多样性和种群遗传结构，我们开发了微卫星引物。

方法和结果

利用序列包含（FIASCO）重复的快速 AFLP 分离（Fast Isolation by AFLP of Sequences Containing，FIASCO）协议，在两个野生种群中鉴定出 15 对引物。每个位点的等位基因数范围为 2 到 5，平均值为 3.3。观察到的和预期的杂合度分别在 0.167 到 0.917 和 0.159 到 0.822 之间变化。所有这些引物都能成功地在近缘种 C. tortisepalum 中扩增，并且有 12 个引物在 C. faberi 和 C. sinense 中也很有用。