新型开发的微卫星标记揭示了杓兰属物种之间存在潜在的杂交现象，以及对杓兰属 C. goeringii 和 C. ensifolium 的种间细分。
The novel developed microsatellite markers revealed potential hybridization among Cymbidium species and the interspecies sub-division of C. goeringii and C. ensifolium.
机构信息
Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, School of Landscape and Architecture, Zhejiang A&F University, Hangzhou, 311300, Zhejiang, China.
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 515005, China.
出版信息
BMC Plant Biol. 2023 Oct 13;23(1):492. doi: 10.1186/s12870-023-04499-y.
BACKGROUND
Orchids (Cymbidium spp.) exhibit significant variations in floral morphology, pollinator relations, and ecological habitats. Due to their exceptional economic and ornamental value, Cymbidium spp. have been commercially cultivated for centuries. SSR markers are extensively used genetic tools for biology identification and population genetics analysis.
RESULT
In this study, nine polymorphic EST-SSR loci were isolated from Cymbidium goeringii using RNA-Seq technology. All nine SSR loci showed transferability in seven other congeneric species, including 51 cultivars. The novel SSR markers detected inter-species gene flow among the Cymbidium species and intra-species sub-division of C. goeringii and C. ensifolium, as revealed by neighborhood-joining and Structure clustering analyses.
CONCLUSION
In this study, we developed nine microsatellites using RNA-Seq technology. These SSR markers aided in detecting potential gene flow among Cymbidium species and identified the intra-species sub-division of C. goeringii and C. ensifolium.
背景
兰花（Cymbidium spp.）在花形态、传粉者关系和生态生境方面表现出显著的变化。由于其特殊的经济和观赏价值，Cymbidium spp. 已经被商业化种植了几个世纪。SSR 标记是生物学鉴定和种群遗传学分析中广泛使用的遗传工具。
结果
本研究利用 RNA-Seq 技术从春兰（Cymbidium goeringii）中分离出 9 个多态性 EST-SSR 位点。这 9 个 SSR 位点在包括 51 个品种在内的其他 7 个同种物种中表现出可转移性。通过邻接聚类和结构聚类分析，新的 SSR 标记揭示了兰花物种之间的种间基因流以及春兰和硬叶兰种内的亚分化。
结论
本研究利用 RNA-Seq 技术开发了 9 个微卫星。这些 SSR 标记有助于检测兰花物种之间的潜在基因流，并鉴定出春兰和硬叶兰的种内亚分化。
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