School of Marine and Tropical Biology, James Cook University, Townsville, QLD 4811, Australia.
Am J Bot. 2011 Apr;98(4):e81-3. doi: 10.3732/ajb.1000356. Epub 2011 Mar 30.
The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified.
Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14.
These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.
第一批微卫星引物是为澳大利亚本土物种中央茄（Solanum centrale）开发的，中央茄被用于丛林食物产业。也扩增了为其他茄属物种开发的现有标记。
使用富集克隆方案，开发了七个新标记，并测试了来自其他茄属物种的 48 个现有标记，结果对中央茄的一组六个高度多态性的共显性位点进行了特征描述。微卫星筛选揭示了多倍体现象。在六个高度多态性的位点中，等位基因多样性范围为 7 到 14。
这些标记将有助于研究遗传多样性，并作为一种简单的方法来估计野生种群的倍性。
