Holinski Sebastian, Knebel Fabian, Heinze Georg, Konertz Wolfgang, Baumann Gert, Borges Adrian C
Department of Cardiovascular Surgery, Charité Campus Mitte, Schumannstr, Berlin, Germany.
Cardiovasc Ultrasound. 2011 May 26;9:15. doi: 10.1186/1476-7120-9-15.
Feasibility of noninvasive monitoring of cardiac function after surgically induced ischemic cardiomyopathy with tissue Doppler and non-Doppler 2D strain echocardiography in rats.
The optimal method for quantitative assessment of global and regional ventricular function in rats with chronic heart failure for research purposes remains unclear.
20 rats underwent suture ligation of the left anterior descending coronary artery via a left thoracotomy to induce ischemic cardiomyopathy. Echocardiographic examination with estimation of left ventricular wall thickness, diameters, fractional shortening, ejection fraction, wall velocities as well as radial strain were performed before and 4 weeks after surgery.
Mean LVEF decreased from 70 ± 6% to 40 ± 8% (p < 0.0001) one month after the operation. LVEDD increased from 7 ± 1 mm to 9 ± 1 mm (p < 0.0001), systolic anterior velocity decreased from 0.79 ± 0.25 cm/s to 0.18 ± 0.19 cm/s (p < 0.0001). Radial 2D strain was significantly reduced after myocardial infarction of the septal (18.2 ± 6.6% vs 7.0 ± 5.9%, p < 0.001), anteroseptal (17.3 ± 5.2% vs 4.6 ± 3.0%, p < 0.0001), anterior (18.9 ± 5.9% vs 5.6 ± 2.5%, p < 0.0001), lateral (21.4 ± 4.9% vs 8.1 ± 3.5%, p < 0.0001) as well as posterior myocardial segments (19.3 ± 5.2% vs 15.4 ± 5.5%, p < 0.01). Inferior segments (19.2 ± 7.9% vs 17.8 ± 7.9%, ns) did not change at all.
It is feasible to assess dimensions, global function, and regional contractility with echocardiography in rats suffering from chronic heart failure after myocardial infarction. Particularly regional function can be exactly evaluated if tissue Doppler and 2D strain is used.
采用组织多普勒和非多普勒二维应变超声心动图对大鼠手术诱导的缺血性心肌病后心脏功能进行无创监测的可行性。
用于研究目的的慢性心力衰竭大鼠左心室整体和局部功能的最佳定量评估方法仍不明确。
20只大鼠通过左胸切口进行左前降支冠状动脉缝扎以诱导缺血性心肌病。在手术前和手术后4周进行超声心动图检查，评估左心室壁厚度、直径、缩短分数、射血分数、壁速度以及径向应变。
术后1个月，平均左心室射血分数从70±6%降至40±8%（p<0.0001）。左心室舒张末期内径从7±1mm增加至9±1mm（p<0.0001），收缩期前向速度从0.79±0.25cm/s降至0.18±0.19cm/s（p<0.0001）。室间隔（18.2±6.6%对7.0±5.9%，p<0.001）、前间隔（17.3±5.2%对4.6±3.0%，p<0.0001）、前壁（18.9±5.9%对5.6±2.5%，p<0.0001）、侧壁（21.4±4.9%对8.1±3.5%，p<0.0001）以及后壁心肌节段（19.3±5.2%对15.4±5.5%，p<0.01）的径向二维应变在心肌梗死后显著降低。下壁节段（19.2±7.9%对17.8±7.9%，无显著性差异）则完全没有变化。
对心肌梗死后慢性心力衰竭大鼠进行超声心动图评估其大小、整体功能和局部收缩性是可行的。如果使用组织多普勒和二维应变，尤其可以准确评估局部功能。
