瘟病毒边境病病毒的细胞内病毒诱导多肽

Intracellular virus-induced polypeptides of pestivirus border disease virus.

作者信息

Akkina R K, Raisch K P

机构信息

Department of Microbiology, College of Veterinary Medicine, Colorado State University, Fort Collins 80523.

出版信息

Virus Res. 1990 Apr;16(1):95-105. doi: 10.1016/0168-1702(90)90046-e.

DOI:10.1016/0168-1702(90)90046-e

PMID:2161595

Abstract

Intracellular virus specific polypeptides of pestivirus, border disease virus (BDV) in bovine turbinate cells were analysed by radio-immunoprecipitation with specific antisera. Eleven viral polypeptides with molecular weights of 220, 165, 118, 84, 66, 58, 55, 53, 45, 37 and 31 kDa, respectively, were detected in infected cells. Of these, the 165, 118, 84, 66, 58, 55, 53, 45 and 31 kDa proteins were found to be glycosylated. Comparative studies indicated that the polypeptides induced by BDV share many antigenic epitopes with those of the polypeptides induced by bovine viral diarrhea virus (BVDV), a serologically related virus of the same genus, pestivirus. The polypeptide profile of BDV appeared to be more similar to that of the noncytopathic BVDV strain NY1 compared to that of cytopathic BVDV strains NADL and Singer. Peptide mapping analysis of homologous polypeptides from BVDV and BDV confirmed their structural relatedness.

摘要

利用特异性抗血清通过放射免疫沉淀法分析了牛鼻甲细胞中瘟病毒属边境病病毒（BDV）的细胞内病毒特异性多肽。在感染细胞中检测到了11种病毒多肽，其分子量分别为220、165、118、84、66、58、55、53、45、37和31 kDa。其中，165、118、84、66、58、55、53、45和31 kDa的蛋白质被发现是糖基化的。比较研究表明，BDV诱导的多肽与瘟病毒属血清学相关病毒牛病毒性腹泻病毒（BVDV）诱导的多肽具有许多抗原表位。与细胞病变性BVDV毒株NADL和Singer相比，BDV的多肽图谱似乎与非细胞病变性BVDV毒株NY1的更相似。对BVDV和BDV同源多肽的肽图分析证实了它们的结构相关性。