Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-806, Republic of Korea.
Syst Appl Microbiol. 2011 Dec;34(8):576-80. doi: 10.1016/j.syapm.2011.03.007. Epub 2011 May 26.
Three halophilic archaea, strains B-1(T), B-3 and B-4, were isolated from evaporitic salt crystals from Namhae, Korea. Cells of the strains were Gram-stain-negative, motile and pleomorphic, and colonies were red-pigmented. The three isolates had identical 16S rRNA gene sequences and formed a tight phylogenetic clade with Halogranum rubrum RO2-11(T) in the genus Halogranum, showing 99.5% sequence similarity. The next most closely related species were Halogranum amylolyticum and Halogranum gelatinilyticum (97.4 and 96.3% similarity to the respective type strains). The phylogeny based on the full-length RNA polymerase subunit B' gene (rpoB') was in agreement with the 16S rRNA gene sequence analysis, but allowed better discrimination. DNA-DNA hybridization between a representative strain (B-1(T)) and the type strains of Hgn. rubrum, Hgn. amylolyticum and Hgn. gelatinilyticum revealed less than 40% relatedness. Polar lipid analysis showed that the three isolates contained phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and three glycolipids. Combined genotypic and phenotypic data supported the conclusion that strains B-1(T), B-3 and B-4 represent a novel species of the genus Halogranum, for which the name Halogranum salarium sp. nov. is proposed. The type strain is B-1(T) (=KCTC 4066(T)=DSM 23171(T)).
从韩国海南蒸发盐晶体中分离到三株嗜盐古菌，分别命名为 B-1(T)、B-3 和 B-4。这些菌株的细胞革兰氏染色阴性，运动且多形性，菌落呈红色。这三个分离株的 16S rRNA 基因序列完全相同，与红盐杆菌 RO2-11(T)在 Halogranum 属中形成紧密的系统发育分支，相似度为 99.5%。其次最接近的物种是嗜淀粉盐杆菌和嗜明胶盐杆菌（与各自的模式菌株相似度分别为 97.4%和 96.3%）。基于全长 RNA 聚合酶亚基 B'（rpoB'）基因的系统发育与 16S rRNA 基因序列分析一致，但具有更好的区分度。代表菌株（B-1(T)）与红盐杆菌、嗜淀粉盐杆菌和嗜明胶盐杆菌的模式菌株之间的 DNA-DNA 杂交显示相似度小于 40%。极性脂分析表明，这三个分离株含有磷脂酰甘油、磷脂酰甘油磷酸甲基酯和三种糖脂。综合表型和基因型数据表明，菌株 B-1(T)、B-3 和 B-4 代表了 Halogranum 属的一个新种，将其命名为 Halogranum salarium sp. nov.。模式菌株为 B-1(T)（=KCTC 4066(T)=DSM 23171(T)）。
