Balage M, Grizard J, Manin M
Laboratoire d'Etude du Métabolisme Azoté, INRA Centre de Clermont-Theix, Ceyrat, France.
Horm Metab Res. 1990 Apr;22(4):207-14. doi: 10.1055/s-2007-1004886.
The effect of specific calorie deprivation was studied in meal-fed growing rats. It resulted in a 50% decrease in growth rate. Blood glucose and most non-essential blood free amino acid levels were depressed. Postprandial plasma insulin was decreased. With insulin ranging from 0.01 to 100 nM, insulin binding to crude Triton X-100 solubilized membranes from liver was higher in calorie restricted rats when compared with control rats. Wheat germ agglutinin (WGA) purified receptor preparations also exhibited higher insulin binding in liver from experimental group but the significance (P less than 0.05) was only visible with low insulin levels; both basal and insulin-stimulated tyrosine-kinase activity were left unchanged. In contrast, whatever the skeletal muscle insulin receptor preparation (enriched plasma membranes, crude Triton X-100 solubilized or wheat-germ agglutinin purified extracts) insulin binding was similar in control and calorie-restricted rats.
在以进食方式喂养的生长大鼠中研究了特定热量剥夺的影响。结果导致生长速率下降50%。血糖和大多数非必需的游离氨基酸水平降低。餐后血浆胰岛素水平下降。当胰岛素浓度在0.01至100 nM范围内时，与对照大鼠相比，热量限制大鼠肝脏中胰岛素与经Triton X - 100粗提物溶解的膜的结合更高。小麦胚凝集素（WGA）纯化的受体制剂在实验组肝脏中也表现出更高的胰岛素结合，但只有在低胰岛素水平时才有统计学意义（P < 0.05）；基础和胰岛素刺激的酪氨酸激酶活性均未改变。相比之下，无论骨骼肌胰岛素受体制剂（富集的质膜、经Triton X - 100粗提物溶解的或经小麦胚凝集素纯化的提取物）如何，对照大鼠和热量限制大鼠的胰岛素结合情况相似。
