Johnson J C, Van Emon J M
United States Environmental Protection Agency, National Exposure Research Laboratory, Characterization Research Division [Formula: see text] Las Vegas, Las Vegas, Nevada 89119.
Anal Chem. 1996 Jan 1;68(1):162-9. doi: 10.1021/ac950410j.
An enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of Aroclors 1242, 1248, 1254, and 1260 in soil and sediments was developed and its performance compared with that of gas chromatography (GC). The detection limits for Aroclors 1242 and 1248 in soil are 10.5 and 9 ng/g, respectively. The assay linear dynamic range is 50-1333 ng/g. Cross-reactivity of the assay with 37 structurally related potential cocontaminants in environmental soil samples was examined; none of the chlorinated anisoles, benzenes, or phenols exhibited >3% cross-reactivity, with <0.1% cross-reactivity being the norm. Soil spike recoveries of 107% and 104% were obtained for Aroclors 1242 and 1248, respectively, for a spike level of 5 mg/kg, with corresponding relative standard deviations of 14% and 17%. One hundred forty-eight environmental soil, sediment, and paper pulp samples, obtained from two EPA listed Superfund sites, were analyzed by ELISA and standard GC methods. Samples were extracted for ELISA analysis by shaking with methanol. Additional extractions of the same samples were performed either with supercritical carbon dioxide or by Soxhlet extraction with methanol. ELISA results for both the supercritical fluid and the Soxhlet extracts were in close agreement with the GC results, while the ELISA results for the methanol shake extracts were not. The data for the environmental samples demonstrated the capability of the ELISA to provide accurate results and reinforced the dependence of any detection method, including ELISA, on appropriate extraction procedures.
开发了一种用于定量测定土壤和沉积物中多氯联苯混合物1242、1248、1254和1260的酶联免疫吸附测定（ELISA）法，并将其性能与气相色谱法（GC）进行了比较。土壤中多氯联苯混合物1242和1248的检测限分别为10.5和9 ng/g。该测定的线性动态范围为50 - 1333 ng/g。检测了该测定法与环境土壤样品中37种结构相关的潜在共污染物的交叉反应性；氯代苯甲醚、苯或酚均未表现出>3%的交叉反应性，通常交叉反应性<0.1%。对于加标水平为5 mg/kg的多氯联苯混合物1242和1248，土壤加标回收率分别为107%和104%，相应的相对标准偏差分别为14%和17%。采用ELISA和标准GC方法对从两个美国环境保护局（EPA）列出的超级基金场地采集的148份环境土壤、沉积物和纸浆样品进行了分析。通过与甲醇振荡进行ELISA分析的样品提取。对相同样品进行了额外的超临界二氧化碳萃取或甲醇索氏提取。超临界流体萃取物和索氏提取物的ELISA结果与GC结果密切一致，而甲醇振荡提取物的ELISA结果则不然。环境样品的数据证明了ELISA能够提供准确结果，并强化了包括ELISA在内的任何检测方法对适当提取程序的依赖性。
