关于14 S亚基在脊髓灰质炎病毒形态发生中前驱作用的新证据。
New evidence for the precursor role of 14 S subunits in poliovirus morphogenesis.
作者信息
Rombaut B, Vrijsen R, Boeyé A
机构信息
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
出版信息
Virology. 1990 Jul;177(1):411-4. doi: 10.1016/0042-6822(90)90502-i.
PMID:2162113
Abstract
In poliovirus-infected HeLa cells incubated as 30 degrees, 14 S subunits are selectively labeled and no virions are assembled. When the temperature is subsequently shifted to 37 degrees, the radioactivity of the 14 S material is transferred mainly to virions, showing that 14 S subunits are virion precursors. When 14 S subunits synthesized at 30 degrees are incubated in vitro at 37 degrees, they assemble to empty capsids.
摘要
在30摄氏度下培养的脊髓灰质炎病毒感染的HeLa细胞中，14S亚基被选择性标记，且没有病毒粒子组装。当温度随后升至37摄氏度时，14S物质的放射性主要转移到病毒粒子上，这表明14S亚基是病毒粒子的前体。当在30摄氏度下合成的14S亚基在37摄氏度下进行体外培养时，它们会组装成空衣壳。
Zotero 插件