MOE Key Laboratory of Environment & Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, People's Republic of China.
Biosens Bioelectron. 2011 Jul 15;26(11):4450-6. doi: 10.1016/j.bios.2011.05.001. Epub 2011 May 11.
A rapid, sensitive and selective electrochemical method was proposed for the determination of 2,4-dinitrophenol (2,4-DNP) in surface water samples, using hydrophilic molecular imprinted polymers (MIPs) as the recognition element and nickel (Ni) fiber as the catalytic element. Hydrophilic MIPs were synthesized using 2,4-DNP as the template, acrylamide as the monomer, glycidilmethacrylate as the pro-hydrophilic co-monomer and acetonitrile as the solvent. Hydrophilic modification could enhance the accessibility of 2,4-DNP to the imprinted cavities and improve the selective recognition properties of traditional MIPs in water medium. Subsequently, hydrophilic MIPs/Ni fiber electrode was prepared to determine trace 2,4-DNP by cyclic voltammetry. The parameters affecting the analytical performance were investigated. Under optimized conditions, the linear range was 0.7-30 μg L⁻¹ and the detection limit was 0.1 μg L⁻¹. Finally, the proposed method was applied to measure 2,4-DNP in surface water samples. The spiked recoveries were changed from 91.3% to 102.6% and the RSD was not higher than 5.1%. There was no statistically significant difference between the results obtained by the proposed method and the traditional chromatographic method.
一种快速、灵敏和选择性的电化学方法被提出，用于测定地表水中的 2,4-二硝基苯酚（2,4-DNP），使用亲水性分子印迹聚合物（MIPs）作为识别元件和镍（Ni）纤维作为催化元件。亲水性 MIPs 是使用 2,4-DNP 作为模板、丙烯酰胺作为单体、甲基丙烯酸缩水甘油酯作为亲水性共聚单体和乙腈作为溶剂合成的。亲水性修饰可以增强 2,4-DNP 进入印迹空穴的可及性，并提高传统 MIPs 在水介质中的选择性识别性能。随后，制备了亲水性 MIPs/Ni 纤维电极，通过循环伏安法测定痕量 2,4-DNP。研究了影响分析性能的参数。在优化条件下，线性范围为 0.7-30μg L⁻¹，检测限为 0.1μg L⁻¹。最后，该方法用于测定地表水中的 2,4-DNP。加标回收率在 91.3%至 102.6%之间变化，相对标准偏差不高于 5.1%。与传统色谱法相比，该方法的结果没有统计学上的显著差异。
