School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China.
Biosens Bioelectron. 2011 Nov 15;29(1):29-33. doi: 10.1016/j.bios.2011.07.058. Epub 2011 Jul 30.
In this paper, an electrochemical sensor for 17β-estradiol (E2) based on the molecular imprinting polymer (MIP) membranes had been constructed. 6-mercaptonicotinic acid (MNA) and E2 were first assembled on the surface of platinum nanoparticles-modified glassy carbon electrode (PtNPs/GCE) by the formation of Pt-S bonds and hydrogen-bonding interactions, and subsequently the polymer membranes were formed by electropolymerization. Finally, a novel molecularly imprinted sensor (MIS) was obtained after removal of E2. Experimental parameters such as deposition time, scan cycles, pH value and accumulation condition were optimized. Under optimal conditions, the MIS exhibited a large adsorption capacity and high selectivity. A good linearity was obtained in the range of 3.0×10(-8)-5.0×10(-5)molL(-1) (r=0.996) with an estimated detection limit of 1.6×10(-8)molL(-1). MIS had been successfully used to analyze E2 in water samples without complex pretreatment. Meanwhile, the average recoveries were higher than 93.9% with RSD<3.7%. All results above reveal that MIS is an effective electrochemical technique to determine E2 real-time in complicated matrix.
本文构建了一种基于分子印迹聚合物（MIP）膜的电化学传感器用于检测 17β-雌二醇（E2）。6-巯基烟酸（MNA）和 E2 首先通过 Pt-S 键和氢键相互作用组装在铂纳米粒子修饰的玻碳电极（PtNPs/GCE）表面，随后通过电化学聚合形成聚合物膜。最后，在去除 E2 后得到了一种新型的分子印迹传感器（MIS）。实验参数如沉积时间、扫描循环、pH 值和积累条件等进行了优化。在最佳条件下，MIS 表现出较大的吸附容量和较高的选择性。在 3.0×10(-8)-5.0×10(-5)molL(-1)（r=0.996）范围内获得了良好的线性关系，检测限估计为 1.6×10(-8)molL(-1)。MIS 已成功用于未经复杂预处理的水样中 E2 的分析。同时，平均回收率高于 93.9%，RSD<3.7%。所有结果均表明，MIS 是一种用于实时检测复杂基质中 E2 的有效电化学技术。
