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用高效液相色谱蒸发光散射和质谱法对环糊精糖基转移酶产生的糖基化烷基聚葡糖苷进行表征。

Characterisation of a glycosylated alkyl polyglycoside produced by a cyclodextrin glycosyltransferase by HPLC-ELSD and -MS.

机构信息

Department of Biotechnology, Lund University, Lund, Sweden.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Jul 1;879(21):1857-60. doi: 10.1016/j.jchromb.2011.04.037. Epub 2011 May 27.

DOI:10.1016/j.jchromb.2011.04.037
PMID:21621486
Abstract

A transglycosylation reaction between an alkyl polyglycoside and α-cyclodextrin catalysed by cyclodextrin glycosyltransferase (CGTase) from Bacillus macerans was investigated. The reaction products were identified by comparison with standards generated by CGTase catalysed modification of pure alkyl glycosides using HPLC-ELSD and -MS analysis. The main products were alkyl glucopyranosides (substrates present in the alkyl polyglycoside) glycosylated with 6 (primary coupling products) or 12 (secondary coupling products) glucose residues. Both α and β anomers were glycosylated.

摘要

研究了脂肪醇聚葡糖苷与α-环糊精在来源于嗜热脂肪芽孢杆菌的环糊精葡萄糖基转移酶（CGTase）催化下的转糖苷反应。通过高效液相色谱-蒸发光散射检测和质谱分析，将反应产物与由 CGTase 催化的纯脂肪糖苷修饰生成的标准品进行比较，对反应产物进行了鉴定。主要产物是用 6 个（初级偶联产物）或 12 个（次级偶联产物）葡萄糖残基糖苷化的葡萄糖基烷基吡喃糖苷（脂肪醇聚葡糖苷中的底物）。两种α和β异构体都发生了糖苷化。

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