Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asan-si, Chungnam, 336-708, Republic of Korea.
J Ind Microbiol Biotechnol. 2013 Dec;40(12):1329-56. doi: 10.1007/s10295-013-1332-0. Epub 2013 Sep 5.
Glycosylation is one of the most important post-modification processes of small molecules and enables the parent molecule to have increased solubility, stability, and bioactivity. Enzyme-based glycosylation has achieved significant progress due to advances in protein engineering, DNA recombinant techniques, exploitation of biosynthetic gene clusters of natural products, and computer-based modeling programs. Our report summarizes glycosylation data that have been published within the past five years to provide an overall review of current progress. We also present the future trends and perspectives for glycosylation.
糖基化是小分子最重要的翻译后修饰过程之一，它使母体分子具有更高的溶解度、稳定性和生物活性。由于蛋白质工程、DNA 重组技术、天然产物生物合成基因簇的开发以及基于计算机的建模程序的进步，基于酶的糖基化已经取得了重大进展。我们的报告总结了过去五年发表的糖基化数据，为当前进展提供了全面的综述。我们还提出了糖基化的未来趋势和展望。
