Ozawa T, Hanaki A
Division of Chemical Pharmacology, National Institute of Radiological Sciences, Chiba-shi, Japan.
Biochem Int. 1990;20(4):649-57.
The first spin-trapping evidence for the formation of thiosulfate (S2O3-.) and sulfide (S-.) radical anions from the reactions of hydrogen peroxide with thiosulphate and sulphide ions, respectively, was presented by electron spin resonance (ESR) spectroscopy using 3,5-dibromo-4-nitrosobenzenesulfonate (DBNBS, 1a) as a spin-trap in aqueous solutions. From the facts that the short-lived radical anions, S2O3-. and S-., could be detected during the oxidation with H2O2, it is suggested that these radical anions may become one of the candidates for the toxicity of sulfide ion in the living body.
通过电子自旋共振（ESR）光谱法，以3,5 - 二溴 - 4 - 亚硝基苯磺酸盐（DBNBS，1a）作为自旋捕获剂，在水溶液中首次获得了自旋捕获证据，证明过氧化氢分别与硫代硫酸盐和硫化物离子反应会形成硫代硫酸根（S2O3-·）和硫离子（S-·）自由基阴离子。基于在H2O2氧化过程中能够检测到短寿命自由基阴离子S2O3-·和S-·这一事实，表明这些自由基阴离子可能是硫化物离子在生物体内产生毒性的候选因素之一。
