超氧阴离子的自旋捕获：水溶性亚硝基芳烃试剂DBNBS使用中的复杂性。
Spin trapping of the superoxide anion: complications in the use of the water-soluble nitroso-aromatic reagent DBNBS.
作者信息
Mani V, Crouch R K
机构信息
Department of Ophthalmology, Medical University of South Carolina, Charleston 29425.
出版信息
J Biochem Biophys Methods. 1989 Mar;18(2):91-6. doi: 10.1016/0165-022x(89)90070-5.
Sodium 3,5-dibromo-4-nitrosobenzenesulfonate (DBNBS) is reported to be a useful spin trap for the measurement of superoxide anions in aqueous solution. However, the signal observed arises from interaction of the spin trap with some species other than the superoxide radical or hydrogen peroxide, a product of its dismutation, as the addition of both superoxide dismutase and catalase to a superoxide generating system failed to attenuate the signal. Therefore caution must be employed in the interpretation of results obtained using this spin trap.
据报道，3,5-二溴-4-亚硝基苯磺酸钠（DBNBS）是一种用于测量水溶液中超氧阴离子的有用自旋捕获剂。然而，观察到的信号源自自旋捕获剂与超氧自由基或其歧化产物过氧化氢以外的某些物质的相互作用，因为向超氧产生系统中添加超氧化物歧化酶和过氧化氢酶均未能减弱该信号。因此，在解释使用这种自旋捕获剂获得的结果时必须谨慎。
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