Acosta Elisa, Avila Julio, Mobasheri Ali, Martin-Vasallo Pablo
Laboratorio de Biologia del Desarrollo, Departamento de Bioquimica y Biologia Molecular, Universidad de La Laguna, La Laguna, Tenerife, Spain.
Front Biosci (Elite Ed). 2011 Jun 1;3(4):1229-40. doi: 10.2741/e326.
The expression of Na+, K+-ATPase alpha and beta subunits isoforms, FXYD2 and FXYD7 were studied in rat adipose stem cell (ASC) by qRT-PCR and immunofluorescence. ASCs were able to differentiate to chondrocytes or adipocytes. All studied genes were expressed in freshly isolated ASCs and in all passages checked. Immunostaining for alpha1 isoform was found in plasma membrane and nuclear envelope, alpha2 signal was lower and alpha3 staining was variable among cells. Beta isoforms signal was abundant and displayed an isoform-specific picture. Staining for FXYD7 was homogeneous in plasma membrane and cytosol. Chondrocytes differenciated from ASC showed identical Na+, K+-ATPase subunits isoforms expression patterns to chondrocytes in cartilage. The expression pattern of Na+, K+-ATPase genes in ASCs exhibits a unique phenotypic signature that implies functional differences in Na+ and K+ transport rates. Furthermore, this phenotypic signature may also be used as a complementary marker for studies of mesenchymal stem cell differentiation. We propose a possible 'moonlighting' role of Na+, K+-ATPase beta isoforms that could be essential for the study of mesenchymal stem cell function and differentiation.
通过定量逆转录聚合酶链反应（qRT-PCR）和免疫荧光法，研究了大鼠脂肪干细胞（ASC）中Na +，K + -ATP酶α和β亚基异构体、FXYD2和FXYD7的表达。脂肪干细胞能够分化为软骨细胞或脂肪细胞。所有研究的基因在新鲜分离的脂肪干细胞以及所有检测的传代细胞中均有表达。在质膜和核膜中发现了α1异构体的免疫染色，α2信号较低，α3染色在细胞间存在差异。β异构体信号丰富，并呈现出异构体特异性的图像。FXYD7在质膜和细胞质中的染色均匀。从脂肪干细胞分化而来的软骨细胞显示出与软骨中的软骨细胞相同的Na +，K + -ATP酶亚基异构体表达模式。脂肪干细胞中Na +，K + -ATP酶基因的表达模式表现出独特的表型特征，这意味着Na +和K +转运速率存在功能差异。此外，这种表型特征也可作为间充质干细胞分化研究的补充标志物。我们提出了Na +，K + -ATP酶β异构体可能的“兼职”作用，这对于间充质干细胞功能和分化的研究可能至关重要。
