Baker Bechmann Marc, Rotoli Deborah, Morales Manuel, Maeso María Del Carmen, García María Del Pino, Ávila Julio, Mobasheri Ali, Martín-Vasallo Pablo
Laboratorio de Biología del Desarrollo, UD de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias, Universidad de La Laguna Santa Cruz de Tenerife, Spain.
Laboratorio de Biología del Desarrollo, UD de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias, Universidad de La LagunaSanta Cruz de Tenerife, Spain; Institute of Endocrinology and Experimental Oncology, National Research CouncilNaples, Italy.
Front Physiol. 2016 Jan 29;7:9. doi: 10.3389/fphys.2016.00009. eCollection 2016.
The goal of this study was to define Na,K-ATPase α and β subunit isoform expression and isozyme composition in colorectal cancer cells and liver metastases. The α1, α3, and β1 isoforms were the most highly expressed in tumor cells and metastases; in the plasma membrane of non-neoplastic cells and mainly in a cytoplasmic location in tumor cells. α1β1 and α3β1 isozymes found in tumor and metastatic cells exhibit the highest and lowest Na(+) affinity respectively and the highest K(+) affinity. Mesenchymal cell isozymes possess an intermediate Na(+) affinity and a low K(+) affinity. In cancer, these ions are likely to favor optimal conditions for the function of nuclear enzymes involved in mitosis, especially a high intra-nuclear K(+) concentration. A major and striking finding of this study was that in liver, metastasized CRC cells express the α3β1 isozyme. Thus, the α3β1 isozyme could potentially serve as a novel exploratory biomarker of CRC metastatic cells in liver.
本研究的目的是确定结直肠癌细胞和肝转移灶中钠钾ATP酶α和β亚基同工型的表达及同工酶组成。α1、α3和β1同工型在肿瘤细胞和转移灶中表达最高；在非肿瘤细胞的质膜中，而在肿瘤细胞中主要位于细胞质中。在肿瘤细胞和转移细胞中发现的α1β1和α3β1同工酶分别表现出最高和最低的钠亲和力以及最高的钾亲和力。间充质细胞同工酶具有中等的钠亲和力和低的钾亲和力。在癌症中，这些离子可能有利于有丝分裂中涉及的核酶功能的最佳条件，尤其是高核内钾浓度。本研究的一个主要且显著的发现是，在肝脏中，转移的结直肠癌细胞表达α3β1同工酶。因此，α3β1同工酶有可能作为肝脏中结直肠癌转移细胞的一种新型探索性生物标志物。
