Okabe T, Yasukawa K
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Cancer Res. 1990 Jul 1;50(13):3863-5.
Human lung cancer cell line, T3M-30, has been shown to produce a growth factor that stimulates proliferation of peripheral blood monocytes. In the presence of this factor, human circulating monocytes were able to proliferate in vitro. Gel exclusion chromatography of the conditioned medium revealed a single peak of monocyte growth-promoting activity at an apparent molecular weight of 16,000. The growth-promoting activity was adsorbed to an anion-exchange column, Mono Q, and eluted with a salt gradient as a single peak of bioactivity at 300 mM NaCl. When the sample was applied to a Vydac C4 column, a reverse-phase high-performance liquid chromatography column, a single peak of activity was observed at a concentration of 76% acetonitrile in 0.1% trifluoroacetic acid. The monocyte growth-promoting activity was heat stable at 56 degrees C. It was partially destroyed by trypsin. The activity was lost after treatment with 2-mercaptoethanol.
人肺癌细胞系T3M - 30已被证明能产生一种刺激外周血单核细胞增殖的生长因子。在这种因子存在的情况下，人循环单核细胞能够在体外增殖。对条件培养基进行凝胶排阻色谱分析，发现在表观分子量为16,000处有一个单核细胞生长促进活性的单峰。生长促进活性被吸附到阴离子交换柱Mono Q上，并以盐梯度洗脱，在300 mM NaCl处作为一个生物活性单峰被洗脱出来。当将样品应用于Vydac C4柱（一种反相高效液相色谱柱）时，在含0.1%三氟乙酸的76%乙腈浓度下观察到一个活性单峰。单核细胞生长促进活性在56℃下热稳定。它被胰蛋白酶部分破坏。用2 - 巯基乙醇处理后活性丧失。
