Okabe T, Fujisawa M, Mihara A, Sato S, Fujiyoshi N, Takaku F
Cancer Res. 1986 Mar;46(3):1043-6.
To examine whether human leukemic cells produce growth factor(s), a protein-free culture line of human erythroleukemic cells (K-562T1) has been established. This unique cell line has been continuously propagated in protein-free Ham's F-10 medium without any supplement for 5 yr. Growth-promoting activity was determined by measuring [3H]thymidine incorporation into DNA in serum-deprived chick embryo fibroblasts. The conditioned medium of K-562T1 contained the growth-promoting activity against chick embryo fibroblasts, mouse 3T3-L1 cells, and K-562 human leukemic cells. This leukemia-derived growth-promoting activity was heat and acid stable and trypsin sensitive. The activity was destroyed by dithiothreitol. Size exclusion chromatography revealed three peaks of activity, with apparent molecular weights of 13,500, 6,300, and 2,400, respectively.
为了检测人类白血病细胞是否产生生长因子，已建立了一种无蛋白培养的人类红白血病细胞系（K-562T1）。这个独特的细胞系已在无蛋白的Ham's F-10培养基中连续传代培养5年，未添加任何补充物。通过测量[3H]胸腺嘧啶核苷掺入血清饥饿的鸡胚成纤维细胞的DNA中来确定生长促进活性。K-562T1的条件培养基对鸡胚成纤维细胞、小鼠3T3-L1细胞和K-562人类白血病细胞具有生长促进活性。这种源自白血病的生长促进活性对热和酸稳定，但对胰蛋白酶敏感。该活性可被二硫苏糖醇破坏。尺寸排阻色谱显示出三个活性峰，其表观分子量分别为13,500、6,300和2,400。
