Department of Genetics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. Institute of Diabetes, Obesity and Metabolism, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Nat Struct Mol Biol. 2011 Jun;18(6):742-6. doi: 10.1038/nsmb.2060. Epub 2011 May 29.
Binding to nucleosomal DNA is critical for 'pioneer' transcription factors such as the winged-helix transcription factors Foxa1 and Foxa2 to regulate chromatin structure and gene activation. Here we report the genome-wide map of nucleosome positions in the mouse liver, with emphasis on transcriptional start sites, CpG islands, Foxa2 binding sites and their correlation with gene expression. Despite the heterogeneity of liver tissue, we could clearly discern the nucleosome pattern of the predominant liver cell, the hepatocyte. By analyzing nucleosome occupancy and the distributions of heterochromatin protein 1 (Hp1), CBP (also known as Crebbp) and p300 (Ep300) in Foxa1- and Foxa2-deficient livers, we find that the maintenance of nucleosome position and chromatin structure surrounding Foxa2 binding sites is independent of Foxa1 and Foxa2.
与核小体 DNA 的结合对于“先驱”转录因子（如翼状螺旋转录因子 Foxa1 和 Foxa2）至关重要，它们可以调节染色质结构和基因激活。在这里，我们报告了在小鼠肝脏中核小体位置的全基因组图谱，重点是转录起始位点、CpG 岛、Foxa2 结合位点及其与基因表达的相关性。尽管肝脏组织存在异质性，但我们可以清楚地分辨出主要的肝脏细胞，即肝细胞的核小体模式。通过分析 Foxa1 和 Foxa2 缺陷型肝脏中的核小体占有率以及异染色质蛋白 1（Hp1）、CBP（也称为 Crebbp）和 p300（Ep300）的分布，我们发现 Foxa2 结合位点周围核小体位置和染色质结构的维持不依赖于 Foxa1 和 Foxa2。
