Reproductive Cell Biology Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.
Am J Reprod Immunol. 2011 Nov;66(5):385-93. doi: 10.1111/j.1600-0897.2011.01017.x. Epub 2011 May 30.
To investigate the role of syndecan-1 in the differentiation of the BeWo cells into syncytiotrophoblast.
BeWo cells were stimulated with forskolin to form syncytia, and the expression of syndecan-1, desmoplakin I+II, human chorionic gonadotrophin (hCG) and angiogenesis-associated factors was analyzed. Syndecan-1 was silenced by siRNA to evaluate its involvement in the forskolin-mediated syncytia formation.
Treatment of the BeWo cells with forskolin led to a significant increase in the syncytia formation. It was associated with an increase in the expression of syndecan-1 with a concomitant decrease in the expression of desmoplakin I+II. Forskolin treatment of the BeWo cells also led to an increase in the secretion of soluble endoglin, whereas no change was observed in the soluble fms-like tyrosine kinase-1. Silencing of the syndecan-1 expression in BeWo cells led to a significant decrease in cell fusion both in the presence and in the absence of forskolin. It was associated with a significant decrease in hCG level in the conditioned medium.
Syndecan-1 is up-regulated in BeWo cells during differentiation and its silencing inhibits syncytialization and thus could be a useful biomarker for syncytiotrophoblast formation.
研究硫酸乙酰肝素蛋白聚糖-1 在 BeWo 细胞向合体滋养层分化中的作用。
用 forskolin 刺激 BeWo 细胞形成合胞体，分析硫酸乙酰肝素蛋白聚糖-1、桥粒芯糖蛋白 I+II、人绒毛膜促性腺激素 (hCG) 和血管生成相关因子的表达。用 siRNA 沉默硫酸乙酰肝素蛋白聚糖-1，以评估其在 forskolin 介导的合胞体形成中的作用。
用 forskolin 处理 BeWo 细胞可显著增加合胞体形成。这与硫酸乙酰肝素蛋白聚糖-1 的表达增加以及桥粒芯糖蛋白 I+II 的表达减少有关。forskolin 处理 BeWo 细胞还导致可溶性内皮素(endoglin)的分泌增加，而可溶性 fms 样酪氨酸激酶-1 则没有变化。BeWo 细胞中硫酸乙酰肝素蛋白聚糖-1 的表达沉默导致合胞体形成在有无 forskolin 的情况下均显著减少。这与条件培养基中 hCG 水平的显著下降有关。
硫酸乙酰肝素蛋白聚糖-1 在 BeWo 细胞分化过程中上调，其沉默抑制了合胞体化，因此可能是合体滋养层形成的有用生物标志物。
