Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Lab of Novel Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, China.
Appl Biochem Biotechnol. 2011 Sep;165(2):719-27. doi: 10.1007/s12010-011-9290-3. Epub 2011 May 31.
Three stress-tolerant phosphate-solubilizing fungal strains identified as Aspergillus niger, Aspergillus japonicus, and Penicillium simplicissimum were isolated from wheat rhizospheric soil. The strains demonstrated different capabilities of phosphate solubilization in National Botanical Research Institute's phosphate medium containing rock phosphate (RP) as sole phosphorus (P) source, and the solubilization of RP by P. simplicissimum was the most effective among these strains, followed by A. niger and A. japonicus. All the strains exhibited high levels of stress tolerance like 10∼45°C temperature, 4∼11 pH, 0∼3.5% NaCl, and 0∼35% PEG 10000. The strains also differed in their abilities to survive and release soluble P from RP under different stresses. A. niger showed significantly higher tolerance to temperature and pH over the other two strains. Higher amount of spores and content of soluble P in the medium were observed in the presence of 3.5% NaCl with P. simplicissimum, followed by A. niger and A. japonicus. P. simplicissimum could not solubilize RP in the presence of 35% PEG 10000, which exhibited the lowest tolerance to desiccation stress among the three strains.
从小麦根际土壤中分离到 3 株耐胁迫溶磷真菌，分别鉴定为黑曲霉、米曲霉和粗糙脉孢菌。这些菌株在含有磷矿粉（RP）作为唯一磷源的国家植物研究所磷酸盐培养基中表现出不同的溶磷能力，其中粗糙脉孢菌对 RP 的溶磷效果最为显著，其次是黑曲霉和米曲霉。所有菌株均表现出较高的耐胁迫能力，如 10∼45°C 温度、4∼11 pH、0∼3.5%NaCl 和 0∼35%PEG 10000。菌株在不同胁迫条件下从 RP 中存活和释放可溶性磷的能力也存在差异。黑曲霉在温度和 pH 方面的耐受性明显高于其他两种菌株。在 3.5%NaCl 存在的情况下，观察到粗糙脉孢菌的孢子数量和培养基中可溶性磷的含量较高，其次是黑曲霉和米曲霉。在 35%PEG 10000 存在的情况下，粗糙脉孢菌不能溶解 RP，其对干燥胁迫的耐受性最低。
