Ghosh R, Banik A K
Department of Chemical Engineering, University of Calcutta, India.
Indian J Exp Biol. 1998 Jul;36(7):688-92.
A mutant strain of Aspergillus niger AB100 was incubated with samples of rock phosphate. Mutation resulted in a greater amount of solubilisation (30 to 35%) as against the parent strain (10 to 15%). The influence of leaching parameters such as ore concentration (pulp density), particle size, initial pH of the medium, temperature, volume of the medium in 250 ml flasks, inoculum concentration and age of inoculum was studied. When low quantity of rock phosphate is applied (0.1%) the solubilisation of phosphorus was optimal (40.5%). Optimum particle size was--200 to 240 mesh, initial pH of the medium 4.0, optimum volume of the fermentation medium 160 ml, time period of incubation was 8 days, inoculum volume was 7.5 ml, and age of inoculum 7 days. The maximum leaching of phosphorus by using these optimum physical parameters is 45 to 50%.
将黑曲霉突变菌株AB100与磷矿样品一起培养。与亲本菌株（10%至15%）相比，突变导致更大程度的溶解（30%至35%）。研究了浸出参数的影响，如矿石浓度（矿浆密度）、粒度、培养基初始pH值、温度、250毫升烧瓶中培养基的体积、接种物浓度和接种物菌龄。当施用少量磷矿（0.1%）时，磷的溶解最为理想（40.5%）。最佳粒度为-200至240目，培养基初始pH值为4.0，发酵培养基最佳体积为160毫升，培养时间为8天，接种物体积为7.5毫升，接种物菌龄为7天。使用这些最佳物理参数时，磷的最大浸出率为45%至50%。
