Giardina C, Serio G, Simone G, Pennella A, Vacca E, Ricco R, Scordari D, Pesce Delfino V
Istituto di Anatomia Patologica, Università degli Studi, Bari.
Boll Soc Ital Biol Sper. 1990 Feb;66(2):135-41.
In this study ten cases of breast infiltrating ductal carcinoma have been considered. In all of them the content of ER has been evaluated by using monoclonal antibodies. Five of them were ER positive and five were ER negative. For the morphometric study ten nuclei of each case have been considered. By using the S.A.M. (Shape Analytical Morphometry) work-station an analytical study of the nuclear shape was performed. The first step was the extraction of fundamental shape which describes the basic shape of original contour without its irregularities. It was obtained by using two parametric equations. The second step was the evaluation of shape asymmetry by S.A.E. (Shape Asymmetry Evaluator). Finally the contour irregularities were evaluated by Fourier analysis. Along with analytical parameters, dimensions (area, perimeter and maximum diameter) were considered too. All obtained data were submitted to univariate statistical analysis (Student's T test) to compare the two groups (ER positive and ER negative tumors). Area, perimeter and maximum diameter were significatively greater in ER negative cases while analytical parameters were not discriminant between the two groups.
本研究纳入了10例乳腺浸润性导管癌病例。所有病例均采用单克隆抗体评估雌激素受体（ER）含量。其中5例为ER阳性，5例为ER阴性。形态计量学研究中，每例选取10个细胞核。利用形状分析形态计量学（S.A.M.）工作站对细胞核形状进行分析研究。第一步是提取基本形状，该形状描述了原始轮廓的基本形状，去除了其不规则性。这是通过使用两个参数方程获得的。第二步是通过形状不对称评估器（S.A.E.）评估形状不对称性。最后通过傅里叶分析评估轮廓不规则性。除了分析参数外，还考虑了尺寸（面积、周长和最大直径）。所有获得的数据均进行单变量统计分析（学生t检验）以比较两组（ER阳性和ER阴性肿瘤）。ER阴性病例的面积、周长和最大直径显著更大，而分析参数在两组之间无鉴别意义。
