Giardina C, Serio G, Caniglia D M, Lettini T, Ricco R, Renzulli G, Pesce Delfino V
Institute of Pathological Anatomy, University of Bari.
Boll Soc Ital Biol Sper. 1994 Oct-Nov;70(10-11):271-9.
Histological grading of oral squamous cell carcinoma (OSCC) is usually evaluated in a semiquantitative way and this causes a low grade of reproducibility of this prognostic evaluation. The aim in this preliminary phase is to investigate the relation between nuclear shape (studied by an analytical procedure) and histological grading to evaluate the weight of nuclear shape and dimension in grading formulation. Nine cases of OSCC were studied (3 cases of G1, G2 and G3 respectively) with 20 nuclei for each case. All the tumors were localized on the lip. Histologic grading according to the Bryne system was performed. Each nucleus was submitted to a morphometrical study by the SAM (Shape Analytical Morphometry) system which, apart from dimensions allows us to evaluate nuclear contour irregularities and nuclear shape asymmetry. Nuclei of squamous cell carcinoma show a progressive increase in dimensions (area, perimeter and diameter) from G1 to G3. Nuclei of G3 carcinoma are characterized by the most irregular shape. Size differences (mean value) are significant to differentiate G1 nuclei from G2 and G3 nuclei while shape parameters are useful in distinguishing G3 nuclei from G1 and G2. Multivariate discriminant analysis showed that by using only nuclear parameters it is possible to correctly classify about 80% of nuclei. This study, using quantitative methods, confirmed nuclear pleomorphism to be the most important feature in grading evaluation.
口腔鳞状细胞癌（OSCC）的组织学分级通常采用半定量方式进行评估，这导致这种预后评估的可重复性较低。在这个初步阶段，目的是研究核形态（通过分析程序研究）与组织学分级之间的关系，以评估核形态和尺寸在分级制定中的权重。研究了9例OSCC（分别为3例G1、G2和G3），每例有20个细胞核。所有肿瘤均位于唇部。按照布赖恩系统进行组织学分级。每个细胞核通过SAM（形状分析形态计量学）系统进行形态计量学研究，该系统除了能测量尺寸外，还能让我们评估核轮廓不规则性和核形状不对称性。鳞状细胞癌的细胞核从G1到G3尺寸（面积、周长和直径）逐渐增大。G3级癌的细胞核形状最不规则。尺寸差异（平均值）对于区分G1细胞核与G2和G3细胞核具有显著意义，而形状参数有助于区分G3细胞核与G1和G2细胞核。多变量判别分析表明，仅使用核参数就有可能正确分类约80%的细胞核。本研究采用定量方法，证实核多形性是分级评估中最重要的特征。
