Jayasena Chathurani S, Trinh Le A, Bronner Marianne
California Institute of Technology, Pasadena, CA 91125, USA.
Gene Expr Patterns. 2011 Oct;11(7):395-400. doi: 10.1016/j.gep.2011.05.002. Epub 2011 May 24.
Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins that are functionally important during vertebrate development. We have generated a zebrafish gene trap line that produces fluorescently tagged Crmp1 protein, which can be dynamically tracked in living fish at subcellular resolution. The results show that Crmp1 is expressed in numerous sites in the developing nervous system. Early expression is apparent in the forebrain, epiphysis, optic tectum and the developing spinal cord. In the larval brain, Crmp1 is expressed in several distinct brain regions, such as the telencephalon, habenula and cerebellum. In addition, it is expressed in the spinal cord in a manner that persists in the larva. The results suggest that this Crmp1 protein trap line offers a powerful tool to track selected neuronal populations at high resolution.
塌陷反应介导蛋白（CRMPs）是一种胞质磷蛋白，在脊椎动物发育过程中具有重要功能。我们构建了一个斑马鱼基因捕获品系，它能产生荧光标记的Crmp1蛋白，该蛋白可在活鱼中以亚细胞分辨率进行动态追踪。结果表明，Crmp1在发育中的神经系统的多个部位表达。早期表达在前脑、松果体、视顶盖和发育中的脊髓中很明显。在幼体大脑中，Crmp1在几个不同的脑区表达，如端脑、缰核和小脑。此外，它在脊髓中的表达方式在幼体中持续存在。结果表明，这个Crmp1蛋白捕获品系为高分辨率追踪选定的神经元群体提供了一个强大的工具。
