Eicher S D, Patterson J A, Rostagno M H
USDA-ARS, Livestock Behavior Research Unit, 125 S. Russell St., West Lafayette, IN 47907, United States.
Vet Immunol Immunopathol. 2011 Aug 15;142(3-4):258-64. doi: 10.1016/j.vetimm.2011.05.014. Epub 2011 May 13.
To determine if β-glucan plus ascorbic acid affects adherence and pathogenicity of Salmonella Dublin and innate immune response in neonatal calves, 20 calves were fed control or supplemented diets (β-glucan, 0.9 g/d, plus ascorbic acid, 500 mg/d) until d 23. On d 21, 5 calves per treatment received 2.4 × 10(8)CFU of S. Dublin orally. S. Dublin spread through intestinal tissues into mesenteric lymph nodes (MLN), spleen, and lung tissues within 48 h. All supplemented calves had less mRNA expression of IL-1 receptor antagonist in liver. Leukocyte cell surface markers changed in lung cells, but not in blood, MLN, or spleen. CD14 in lungs was greatest for calves receiving supplement and challenge, but CD18 in lungs was greater for challenged than control calves. Lung DEC205 was greatest for challenged calves with and without supplement compared to controls, but more lung cells expressed CD14 for all treated groups compared to controls. These data show that S. Dublin briefly inhabited the intestinal tract, moving quickly to spleen, MLN, and lung tissues. Lung tissue was modulated by S. Dublin, but supplement alone increased CD14 expressing cells. The supplement appears not to attenuate invasiness but modified some lung cell populations by 48h.
为了确定β-葡聚糖加抗坏血酸是否会影响都柏林沙门氏菌在新生犊牛中的黏附性和致病性以及先天免疫反应，20头犊牛被饲喂对照日粮或补充日粮（β-葡聚糖，0.9克/天，加抗坏血酸，500毫克/天）直至第23天。在第21天，每个处理组的5头犊牛经口接种2.4×10⁸CFU的都柏林沙门氏菌。都柏林沙门氏菌在48小时内通过肠道组织扩散到肠系膜淋巴结（MLN）、脾脏和肺组织。所有补充组犊牛肝脏中白细胞介素-1受体拮抗剂的mRNA表达均较低。肺细胞中的白细胞表面标志物发生了变化，但血液、MLN或脾脏中的未变。接受补充日粮并受到攻击的犊牛肺中的CD14最高，但受到攻击的犊牛肺中的CD18比对照犊牛更高。与对照相比，无论是否补充日粮，受到攻击的犊牛肺中的DEC205最高，但与对照相比，所有处理组的肺细胞中表达CD14的更多。这些数据表明，都柏林沙门氏菌短暂定殖于肠道，迅速转移至脾脏、MLN和肺组织。肺组织受到都柏林沙门氏菌的调节，但单独补充日粮增加了表达CD14的细胞。补充日粮似乎并未减弱侵袭性，但在48小时内改变了一些肺细胞群体。
