α -降钙素基因相关肽（CGRP）和β -降钙素基因相关肽的信使核糖核酸（mRNAs）在大鼠脊髓和背根神经节中受到不同的调节。

Alpha-CGRP and beta-CGRP mRNAs are differentially regulated in the rat spinal cord and dorsal root ganglion.

作者信息

Noguchi K, Senba E, Morita Y, Sato M, Tohyama M

机构信息

Department Anatomy II, Osaka University Medical School, Japan.

出版信息

Brain Res Mol Brain Res. 1990 May;7(4):299-304. doi: 10.1016/0169-328x(90)90080-w.

DOI:10.1016/0169-328x(90)90080-w

PMID:2163005

Abstract

We found an increase in calcitonin gene-related peptide (CGRP)-like immunoreactivity in motoneurons of rat spinal cord after peripheral axotomy. By means of in situ hybridization histochemistry and Northern blotting, we further demonstrated that this increase was the result of increased levels of alpha-CGRP mRNA, not beta-CGRP mRNA. The increased level of alpha-CGRP mRNA was maintained for at least 5 weeks, and was present on both sides. In addition, alpha-CGRP and beta-CGRP mRNAs had different distributions from each other in the dorsal root ganglia and levels of both were decreased after axotomy. These results indicate that alpha-CGRP and beta-CGRP are regulated independently and have different roles in the motor and sensory systems of the spinal cord.

摘要

我们发现，外周轴突切断后，大鼠脊髓运动神经元中降钙素基因相关肽（CGRP）样免疫反应性增加。通过原位杂交组织化学和Northern印迹法，我们进一步证明，这种增加是α-CGRP mRNA水平升高的结果，而非β-CGRP mRNA。α-CGRP mRNA水平升高至少维持5周，且双侧均有。此外，α-CGRP和β-CGRP mRNA在背根神经节中的分布彼此不同，轴突切断后两者水平均降低。这些结果表明，α-CGRP和β-CGRP受到独立调节，在脊髓的运动和感觉系统中具有不同作用。