Soyer Tutku, Aliefendioğlu Didem, Aktuna Zuhal, Cağlayan Osman, Aydos Tolga Reşat, Cakmak Murat
Department of Pediatric Surgery, Medical Faculty, Kirikkale University, Kirikkale, Turkey.
Pediatr Surg Int. 2011 Nov;27(11):1197-202. doi: 10.1007/s00383-011-2934-0. Epub 2011 Jun 1.
To evaluate the effect of phototherapy on gastrointestinal smooth muscle activity and oxidative stress.
Wistar albino rats (n = 18, in the first 7 days of life) weighing 7 ± 2 g with both sexes were included in the study. The animals were randomized into three groups. In control group (CG), median laparotomy was performed to obtain 1 cm of jejunum, terminal ileum and colonic segments. In the phototherapy group (PTG), led phototherapy with a wave density of 40 μw/cm(2)/nm were used (Bilitron 3006, Fanem, Brasil). The efficacy surface of phototherapy was 30-40 cm and the exposure distance was 30 cm. The duration of phototherapy was 24 h. Sham group (SG) received white light with the same wave density and exposure distance. The oxidative stress markers and contraction responses were investigated from intestinal segments obtained from experiments.
The jejunum segments showed significantly lowered contraction response to carbachol in SG when compared to CG and PTG (p < 0.05). Decreased contractile response to KCl was detected in both SG and PTG in terminal ileum segments. MDA levels showed no difference between groups (p > 0.05). Total sulfhydryl (T-SH) levels were found significantly increased in PTG when compared to CG and SG (p < 0.05). When NO levels were evaluated, NO levels were found decreased in PTG and SG with respect to CG (p < 0.05).
PT may cause various alterations in oxidant/antioxidant system in intestinal segments. Unlike to clinical findings, decreased contractile responses were detected in rat gastrointestinal smooth muscles after PT.
评估光疗对胃肠道平滑肌活动及氧化应激的影响。
选取出生后7天内、体重7±2克的Wistar白化大鼠（n = 18，雌雄不限）纳入本研究。将动物随机分为三组。对照组（CG）行正中剖腹术获取1厘米的空肠、回肠末端及结肠段。光疗组（PTG）采用波长密度为40μw/cm(2)/nm的LED光疗（Bilitron 3006，Fanem，巴西）。光疗的有效面积为30 - 40厘米，照射距离为30厘米。光疗持续时间为24小时。假手术组（SG）接受相同波长密度和照射距离的白光。从实验获取的肠段中研究氧化应激标志物及收缩反应。
与CG组和PTG组相比，SG组空肠段对卡巴胆碱的收缩反应显著降低（p < 0.05）。在回肠末端段，SG组和PTG组对氯化钾的收缩反应均降低。各组间丙二醛水平无差异（p > 0.05）。与CG组和SG组相比，PTG组总巯基（T - SH）水平显著升高（p < 0.05）。评估一氧化氮水平时，发现PTG组和SG组相对于CG组一氧化氮水平降低（p < 0.05）。
光疗可能导致肠段氧化/抗氧化系统发生多种改变。与临床发现不同，光疗后大鼠胃肠道平滑肌的收缩反应降低。
