Tanaka Keiichiro, Hashimoto Hisashi, Tachibana Toshiaki, Ishikawa Hiroshi, Ohki Takao
Department of Surgery, Jikei University School of Medicine, 3-25-8, Nishi-Shinbashi, Minato-ku, Tokyo 105-8461, Japan.
Pediatr Surg Int. 2008 Jul;24(7):837-42. doi: 10.1007/s00383-008-2170-4. Epub 2008 May 10.
Phototherapy is the most frequently used treatment for the neonatal jaundice. However, recent papers report that phototherapy increased apoptosis in peripheral mononuclear leukocytes in vivo and in mouse lymphoma cell line in vitro. We have investigated the cytotoxicity of phototherapy on the small intestine of neonatal rat using conventional halogen-quartz device (conventional device) and blue light-emitting device (LED device) by measuring apoptotic cells. Four-day-old male Wistar rats were divided into three groups as follows: group 1, exposure to conventional device for 72 h; group 2, exposure to LED device for 72 h; and group 3, control (without phototherapy). After light exposure, the small intestine was examined for apoptosis. Apoptotic cells were detected by the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) assay, by immunohistochemistry for caspase-3 and by transmission electron microscopy. The proportion of positive cells by the TUNEL method in the epithelium of the small intestine was 6.2, 3.1 and 1.7% in the conventional device group, the LED device group and the control group, respectively. The apoptotic cells of the conventional device group is significantly higher than the LED device group (P < 0.01) and that of the LED device group was higher than that of the control group (P < 0.05). We suspected that phototherapy induced apoptosis in neonatal small intestine and the conventional device introduces more apoptosis than the LED device.
光疗是新生儿黄疸最常用的治疗方法。然而，最近的论文报道光疗在体内会增加外周血单核白细胞的凋亡，在体外会增加小鼠淋巴瘤细胞系的凋亡。我们使用传统的卤素石英装置（传统装置）和蓝光发光装置（LED装置），通过测量凋亡细胞，研究了光疗对新生大鼠小肠的细胞毒性。将4日龄雄性Wistar大鼠分为三组：第1组，暴露于传统装置72小时；第2组，暴露于LED装置72小时；第3组，对照组（无光疗）。光照后，检查小肠的凋亡情况。通过TUNEL（末端脱氧核苷酸转移酶介导的dUTP缺口末端标记）检测、caspase-3免疫组化和透射电子显微镜检测凋亡细胞。传统装置组、LED装置组和对照组小肠上皮中TUNEL法阳性细胞比例分别为6.2%、3.1%和1.7%。传统装置组的凋亡细胞明显高于LED装置组（P<0.01），LED装置组的凋亡细胞高于对照组（P<0.05）。我们怀疑光疗会诱导新生大鼠小肠凋亡，且传统装置比LED装置诱导更多凋亡。
