Ewetz L, Thore A
Appl Environ Microbiol. 1978 Dec;36(6):790-3. doi: 10.1128/aem.36.6.790-793.1978.
Protohemin and covalently bound hemin were determined in eight aerobic bacterial strains. A good correlation between protohemin content and luminol reactivity was found. The ratio of luminol reaction to protohemin for the eight investigated strains was essentially identical to that of pure protohemin, 0.7 X 10(16) mV/mol. Covalently bound hemin contributed to the chemiluminescence to a minor extent only (0.7 X 10(14) mV/mol, in accordance with earlier observations of the lower reactivity of cytochrome c and related compounds. A difference in reaction kinetics of the luminol reaction with covalently bound hemin (slower reaction than protohemin) and protohemin was observed in vivo as well as in vitro. The phenomenon could be used to differentiate between strains with different hemin composition.
在八种需氧细菌菌株中测定了原卟啉和共价结合的血红素。发现原卟啉含量与鲁米诺反应性之间具有良好的相关性。所研究的八种菌株的鲁米诺反应与原卟啉的比率与纯原卟啉的比率基本相同，为0.7×10(16) mV/mol。共价结合的血红素对化学发光的贡献仅为很小一部分（0.7×10(14) mV/mol），这与早期关于细胞色素c及相关化合物反应性较低的观察结果一致。在体内和体外均观察到鲁米诺反应与共价结合的血红素（反应比原卟啉慢）和原卟啉的反应动力学存在差异。该现象可用于区分具有不同血红素组成的菌株。
