Shu Lu, Zhu Jinkun, Wang Qingjiang, He Pingang, Fang Yuzhi
Department of Chemistry, East China Normal University, Shanghai, 200062, People's Republic of China.
Luminescence. 2014 Sep;29(6):579-85. doi: 10.1002/bio.2584. Epub 2013 Sep 30.
Based on the catalytic activity of hemin, an efficient biocatalyst, an indirect capillary electrophoresis-chemiluminescence (CE-CL) detection method for phenols using a hemin-luminol-hydrogen peroxide system was developed. Through a series of static injection experiments, hemin was found to perform best in a neutral solution rather than an acidic or alkaline medium. Although halide ions such as Br(-) and F(-) could further enhance the CL signal catalyzed by hemin, it is difficult to apply these conditions to this CE-CL detection system because of the self-polymerization of hemin, as it hinders the CE process. The addition of concentrated ammonium hydroxide to an aqueous/dimethyl sulfoxide solution of hemin-luminol afforded a stable CE-CL baseline. The indirect CE-CL detection of five phenols using this method gave the following limits of detections: 4.8 × 10(-8) mol/L (o-sec-butylphenol), 4.9 × 10(-8) mol/L (o-cresol), 5.4 × 10(-8) mol/L (m-cresol), 5.3 × 10(-8) mol/L (2,4-dichlorophenol) and 7.1 × 10(-8) mol/L (phenol).
基于高效生物催化剂血红素的催化活性，开发了一种使用血红素-鲁米诺-过氧化氢体系的间接毛细管电泳-化学发光（CE-CL）法检测酚类物质。通过一系列静态进样实验发现，血红素在中性溶液中表现最佳，而非酸性或碱性介质。尽管诸如Br(-)和F(-)等卤离子可进一步增强血红素催化的化学发光信号，但由于血红素的自聚合会阻碍毛细管电泳过程，因此难以将这些条件应用于该CE-CL检测系统。向血红素-鲁米诺的水/二甲基亚砜溶液中加入浓氢氧化铵可得到稳定的CE-CL基线。使用该方法对5种酚类物质进行间接CE-CL检测，得到如下检测限：4.8×10(-8) mol/L（邻仲丁基苯酚）、4.9×10(-8) mol/L（邻甲酚）、5.4×10(-8) mol/L（间甲酚）、5.3×10(-8) mol/L（2,4-二氯苯酚）和7.1×10(-8) mol/L（苯酚）。
