Adiotomre J, Eastwood M A, Edwards C A, Brydon W G
Gastro-Intestinal Unit, Western General Hospital, Edinburgh, Scotland.
Am J Clin Nutr. 1990 Jul;52(1):128-34. doi: 10.1093/ajcn/52.1.128.
Gravimetric measurement of dietary fiber (DF) gives no indication of the biological function of any particular fiber. This study describes simple methods based on dialysis and fermentation that enable a hierarchy of fibers to be described for each of the major actions of fiber along the gastro-intestinal tract: nutrient absorption, sterol metabolism, cecal fermentation, and fecal bulking. These results were compared with previous metabolic studies with the same fiber isolates in humans. DF that modifies nutrient absorption can be identified by using dialysis studies, whereas identifying DF that modifies sterol metabolism, cecal fermentation, and fecal weight requires formulas that incorporate dialysis and fermentation results. Results from dialysis and fermentation predicted the action of wheat bran, pectin, guar, gum arabic, carboxymethylcellulose, gellan, tragacanth, xanthan, and karaya in humans and generated anomalous results for karaya and tragacanth. These methods could form the basis of techniques that would enable a screening of novel and processed fibers before studies in animals, including humans.
膳食纤维（DF）的重量法测量无法表明任何特定纤维的生物学功能。本研究描述了基于透析和发酵的简单方法，这些方法能够针对纤维在胃肠道中的每种主要作用（营养吸收、固醇代谢、盲肠发酵和粪便体积增大）对纤维进行分级描述。将这些结果与之前在人体中对相同纤维分离物进行的代谢研究结果进行了比较。可通过透析研究来鉴定能改变营养吸收的膳食纤维，而鉴定能改变固醇代谢、盲肠发酵和粪便重量的膳食纤维则需要结合透析和发酵结果的公式。透析和发酵的结果预测了麦麸、果胶、瓜尔豆胶、阿拉伯胶、羧甲基纤维素钠、结冷胶、黄芪胶、黄原胶和刺梧桐树胶在人体中的作用，并得出了刺梧桐树胶和黄芪胶的异常结果。这些方法可成为相关技术的基础，这些技术能够在包括人体在内的动物研究之前对新型纤维和加工纤维进行筛选。
