Department of General Surgery, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
World J Gastroenterol. 2011 May 28;17(20):2572-9. doi: 10.3748/wjg.v17.i20.2572.
To investigate the proteins involved in colonic adaptation and molecular mechanisms of colonic adaptation in rats with ultra-short bowel syndrome (USBS).
Sprague Dawley rats were randomly assigned to three groups: USBS group (10 rats) undergoing an approximately 90%-95% small bowel resection; sham-operation group (10 rats) undergoing small bowel transaction and anastomosis; and control group (ten normal rats). Colon morphology and differential protein expression was analyzed after rats were given post-surgical enteral nutrition for 21 d. Protein expression in the colonic mucosa was analyzed by two-dimensional electrophoresis (2-DE) in all groups. Differential protein spots were detected by ImageMaster 2D Platinum software and were further analyzed with matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight-mass spectrometric (MALDI-TOF/TOF-MS) analysis.
The colonic mucosal thickness significantly increased in the USBS group compared with the control group (302.1 ± 16.9 μm vs 273.7 ± 16.0 μm, P < 0.05). There was no statistically significant difference between the sham-operation group and control group (P > 0.05). The height of colon plica markedly improved in USBS group compared with the control group (998.4 ± 81.2 μm vs 883.4 ± 39.0 μm, P < 0.05). There was no statistically significant difference between the sham-operation and control groups (P > 0.05). A total of 141 differential protein spots were found in the USBS group. Forty-nine of these spots were down-regulated while 92 protein spots were up-regulated by over 2-folds. There were 133 differential protein spots in USBS group. Thirty of these spots were down-regulated and 103 were up-regulated. There were 47 common differential protein spots among the three groups, including 17 down-regulated protein spots and 30 up-regulated spots. Among 47 differential spots, eight up-regulated proteins were identified by MALDI-TOF/TOF-MS. These proteins were previously reported to be involved in sugar and fat metabolism, protein synthesis and oxidation reduction, which are associated with colonic adaption.
Eight proteins found in this study play important roles in colonic compensation and are associated with sugar and fat metabolism, protein synthesis, and molecular chaperoning.
研究超短肠综合征（USBS）大鼠结肠适应性相关蛋白及其分子机制。
SD 大鼠随机分为三组：USBS 组（10 只），行近 90%-95%小肠切除术；假手术组（10 只），行小肠部分切除及端端吻合术；对照组（10 只），正常饲养。术后给予肠内营养 21 d 后观察各组大鼠结肠形态学变化及差异蛋白表达。采用二维电泳（2-DE）技术比较各组大鼠结肠黏膜差异蛋白表达，用 ImageMaster 2D Platinum 软件分析差异蛋白点，基质辅助激光解吸电离-飞行时间/飞行时间质谱（MALDI-TOF/TOF-MS）分析鉴定差异蛋白。
与对照组相比，USBS 组大鼠结肠黏膜厚度显著增加（302.1±16.9μm 比 273.7±16.0μm，P＜0.05），但假手术组与对照组间差异无统计学意义（P＞0.05）。与对照组相比，USBS 组大鼠结肠黏膜皱襞高度显著增加（998.4±81.2μm 比 883.4±39.0μm，P＜0.05），假手术组与对照组间差异无统计学意义（P＞0.05）。USBS 组共检测到 141 个差异蛋白点，其中 49 个蛋白点表达下调，92 个蛋白点表达上调 2 倍以上；假手术组共检测到 133 个差异蛋白点，其中 30 个蛋白点表达下调，103 个蛋白点表达上调；3 组间有 47 个共同差异蛋白点，包括 17 个下调蛋白点和 30 个上调蛋白点。通过 MALDI-TOF/TOF-MS 鉴定出 8 个差异蛋白点，这些蛋白点与糖脂代谢、蛋白质合成与氧化还原等相关。
本研究发现的 8 个差异表达蛋白在结肠代偿中发挥重要作用，与糖脂代谢、蛋白质合成及分子伴侣等有关。
