Department of Environmental Science and Engineering, Ewha Womans University, 11-1 Daehyun-dong, Seodaemun-gu, Seoul, 120-750, South Korea.
J Ind Microbiol Biotechnol. 2012 Jan;39(1):19-25. doi: 10.1007/s10295-011-0994-8. Epub 2011 Jun 2.
Phospholipid fatty acid (PLFA) profiles in four full-scale activated sludge reactors (ASR1 ~ 4) treating municipal wastewater, South Korea, were monitored to evaluate the influence of influent water quality on microbial community structure (MCS) and the effect of the MCS on effluent water quality. In ASR1 ~ 3, PLFA profiles were very similar, regardless of the influent water quality and seasonal differences, and 16:17c/15:0iso2OH and 16:0 were dominant. PLFA profiles in ASR4 during summer and autumn were very similar to those in ASR1 ~ 3, but increases in specific fatty acids, 16:1ω5c, 11methyl18:1ω7c and 15:0iso3OH, were found in ASR4 during winter and spring, with relatively high total suspended solid (TSS) concentrations in the effluent. 16:1ω5c and 15:0iso3OH, possibly related with Flexibacter sp., caused a bulking problem in the activated sludge. The community diversity indices such as Shannon diversity and equability decreased in summer but increased in autumn in all the ASRs. Canonical correspondence analysis results suggested that the influent BOD concentration played the most important role in changing MCS, followed by influent TSS concentration. In addition, the TSS and total phosphorus concentrations in the effluent were significantly affected by the change of the MCS.
在韩国的四个全规模活性污泥反应器（ASR14）中监测了磷脂脂肪酸（PLFA）谱，以评估进水水质对微生物群落结构（MCS）的影响，以及 MCS 对出水水质的影响。在 ASR13 中，PLFA 谱非常相似，无论进水水质和季节变化如何，16:17c/15:0iso2OH 和 16:0 都是优势种。ASR4 中夏季和秋季的 PLFA 谱与 ASR1~3 非常相似，但在冬季和春季，ASR4 中发现了特定脂肪酸 16:1ω5c、11methyl18:1ω7c 和 15:0iso3OH 的增加，同时出水的总悬浮固体（TSS）浓度相对较高。16:1ω5c 和 15:0iso3OH 可能与 Flexibacter sp. 有关，导致活性污泥发生膨胀问题。所有 ASR 的群落多样性指数（如 Shannon 多样性和均度）在夏季下降，但在秋季增加。典范对应分析结果表明，进水 BOD 浓度对改变 MCS 起着最重要的作用，其次是进水 TSS 浓度。此外，出水的 TSS 和总磷浓度受到 MCS 变化的显著影响。
