Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Sloneczna 45G, 10-709, Olsztyn, Poland.
Environ Sci Pollut Res Int. 2018 Jan;25(2):1655-1669. doi: 10.1007/s11356-017-0615-9. Epub 2017 Nov 3.
By modification of the operational conditions of batch reactors, a municipal wastewater treatment plant was upgraded from activated sludge to aerobic granular sludge (AGS) technology. After upgrading, the volume of the biological reactors was reduced by 30%, but the quality of the effluent substantially improved. The concentration of biomass in the reactors increased twofold; the average biomass yield was 0.6 g MLVSS/g COD, and excess granular sludge was efficiently stabilized in aerobic conditions. Canonical correspondence analysis based on the results of next-generation sequencing showed that the time of adaptation significantly influenced the microbial composition of the granules. In mature granules, the abundance of ammonium-oxidizing bacteria was very low, while the abundance of the nitrite-oxidizing bacteria Nitrospira sp. was 0.5 ± 0.1%. The core genera were Tetrasphaera, Sphingopyxis, Dechloromonas, Flavobacterium, and Ohtaekwangia. Bacteria belonging to these genera produce extracellular polymeric substances, which stabilize granule structure and accumulate phosphorus. The results of this study will be useful for designers of AGS wastewater treatment plants, and molecular data given here provide insight into the ecology of mature aerobic granules from a full-scale facility.
通过改变分批式反应器的操作条件，将城市污水处理厂从活性污泥法升级为好氧颗粒污泥（AGS）技术。升级后，生物反应器的体积减少了 30%，但出水水质得到了显著改善。反应器中生物量的浓度增加了一倍；平均生物量产率为 0.6 g MLVSS/g COD，过量的颗粒污泥在好氧条件下得到有效稳定。基于下一代测序结果的典范对应分析表明，适应时间显著影响了颗粒的微生物组成。在成熟的颗粒中，氨氧化菌的丰度非常低，而亚硝酸盐氧化菌 Nitrospira sp. 的丰度为 0.5±0.1%。核心属是 Tetrasphaera、Sphingopyxis、Dechloromonas、Flavobacterium 和 Ohtaekwangia。属于这些属的细菌产生胞外聚合物，稳定颗粒结构并积累磷。本研究的结果将对 AGS 污水处理厂的设计者有用，此处给出的分子数据深入了解了来自实际规模设施的成熟好氧颗粒的生态学。
