Postgraduate and Research Division, International Medical University, Kuala Lumpur, Malaysia.
Inflamm Res. 2011 Oct;60(10):897-907. doi: 10.1007/s00011-011-0349-y. Epub 2011 Jun 2.
The study investigated the effect of collagen-induced arthritis in Dark Agouti (DA) rats on the level of C-reactive protein and inflammatory cytokine tumour necrosis factor-alpha (TNF-α).
Female Dark Agouti (DA) rats.
Three different dosages of (2 mg/kg of body weight, 3 mg/kg of body weight and 4 mg/kg of body weight) collagen and complete Freund's adjuvant suspension were tested. After 45 days, serum C-reactive protein, TNF-α, superoxide dismutase and total glutathione assays were done. Radiographic and histopathological changes in the joints were compared.
All three groups showed signs of arthritic changes, confirmed by histopathological and radiographic changes. Severe arthritic changes were seen in the rats injected with 4 mg/kg of body weight of collagen. There was a significant increase in C-reactive protein, TNF-α, super oxide dismutase and total glutathione levels in the plasma in arthritis rats and the changes were more significant with 4 mg/kg of collagen.
These results demonstrated that the optimal dose to inject to experimental animals in order to get server arthritic changes was 4 mg/kg of collagen with complete Freund's adjuvant suspension. Severe arthritis changes induced significant elevation in plasma C-reactive protein and TNF-α levels.
研究胶原蛋白诱导的 Dark Agouti（DA）大鼠关节炎对 C 反应蛋白和炎症细胞因子肿瘤坏死因子-α（TNF-α）水平的影响。
雌性 Dark Agouti（DA）大鼠。
测试了三种不同剂量（2 mg/kg 体重、3 mg/kg 体重和 4 mg/kg 体重）的胶原蛋白和完全弗氏佐剂混悬液。45 天后，进行血清 C 反应蛋白、TNF-α、超氧化物歧化酶和总谷胱甘肽测定。比较关节的放射学和组织病理学变化。
所有三组均出现关节炎变化的迹象，通过组织病理学和放射学变化得到证实。注射 4 mg/kg 体重胶原蛋白的大鼠出现严重的关节炎变化。关节炎大鼠血浆中 C 反应蛋白、TNF-α、超氧化物歧化酶和总谷胱甘肽水平显著升高，而 4 mg/kg 胶原蛋白组的变化更为显著。
这些结果表明，为了获得严重的关节炎变化，向实验动物注射的最佳剂量为 4 mg/kg 胶原蛋白与完全弗氏佐剂混悬液。严重的关节炎变化导致血浆 C 反应蛋白和 TNF-α水平显著升高。
