Huang C J, Barrett E L
Department of Food Science and Technology, University of California, Davis 95616.
J Bacteriol. 1990 Jul;172(7):4100-2. doi: 10.1128/jb.172.7.4100-4102.1990.
Transposon Tn5 insertions causing anaerobic cysteine auxotrophy were isolated from a Salmonella typhimurium cysI parent (auxotrophic under aerobic but not anaerobic conditions). Insertions in one mutant group appeared to be in cysG. A second group of insertions, designated asr (anaerobic sulfite reduction), were located near map unit 53 on the S. typhimurium chromosome. They did not cause aerobic or anaerobic auxotrophy in a cys1+ background but did prevent dissimilatory sulfite reduction. Plasmids containing asr DNA cloned from wild-type S. typhimurium conferred anaerobic prototrophy and the ability to produce hydrogen sulfide from sulfite on an Escherichia coli cys1 mutant.
从鼠伤寒沙门氏菌cysI亲本（在需氧条件下为营养缺陷型，但在厌氧条件下不是）中分离出导致厌氧半胱氨酸营养缺陷的转座子Tn5插入。一个突变组中的插入似乎位于cysG中。第二组插入，指定为asr（厌氧亚硫酸盐还原），位于鼠伤寒沙门氏菌染色体上图谱单位53附近。它们在cys1+背景下不会导致需氧或厌氧营养缺陷，但确实会阻止异化亚硫酸盐还原。从野生型鼠伤寒沙门氏菌克隆的含有asr DNA的质粒赋予大肠杆菌cys1突变体厌氧原养型以及从亚硫酸盐产生硫化氢的能力。
