Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.
Phytother Res. 2012 Feb;26(2):214-22. doi: 10.1002/ptr.3533. Epub 2011 Jun 2.
The study evaluated the protective effects of purple rice (Oryza sativa L.) bran extract (PRE) and its constituents, cyanidin and peonidin, against angiogenesis induced by vascular endothelial growth factor (VEGF). The effects of VEGF and PRE were examined by in vitro tube formation assays and following 14-day co-culture of human umbilical vein endothelial cells (HUVECs) and fibroblasts. The antiangiogenic mechanism of PRE was evaluated by VEGF-induced proliferation and migration of HUVECs and/or human retinal microvascular endothelial cells (HRMECs) and phosphorylation of extracellular signal-regulated kinase (ERK) and p38. The PRE significantly suppressed VEGF-induced tube formation, proliferation and migration in HUVECs and HRMECs as well as phosphorylation of ERK and p38. Cyanidin and peonidin also suppressed the proliferation and migration induced by VEGF. These findings indicate that PRE and anthocyanidins suppress VEGF-induced angiogenesis by inhibiting proliferation and migration and suggest that the inhibition of phosphorylated-ERK and -p38 may be involved in the underlying mechanism.
该研究评估了紫米（Oryza sativa L.）糠提取物（PRE）及其成分矢车菊素和芍药素对血管内皮生长因子（VEGF）诱导的血管生成的保护作用。通过体外管形成测定法以及人脐静脉内皮细胞（HUVEC）和成纤维细胞 14 天共培养，研究了 VEGF 和 PRE 的作用。通过 VEGF 诱导的 HUVEC 和/或人视网膜微血管内皮细胞（HRMEC）增殖和迁移以及细胞外信号调节激酶（ERK）和 p38 的磷酸化，评估了 PRE 的抗血管生成机制。PRE 显著抑制了 VEGF 诱导的 HUVEC 和 HRMEC 中的管形成、增殖和迁移，以及 ERK 和 p38 的磷酸化。矢车菊素和芍药素也抑制了 VEGF 诱导的增殖和迁移。这些发现表明，PRE 和花青素通过抑制增殖和迁移来抑制 VEGF 诱导的血管生成，并提示磷酸化-ERK 和 -p38 的抑制可能参与潜在的机制。
